Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-KMT1C/G9a Polyclonal Antibody, Unconjugated(bs-9118R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-9118R
- 2025年10月16日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-9118R |
| 英文名称 | KMT1C Rabbit pAb |
| 中文名称 | 组蛋白H3赖氨酸9甲基化1C抗体 |
| 英文别名 | Bat 8; Bat8; EC 2.1.1.43; EHMT 2; EHMT2; Euchromatic histone lysine N methyltransferase 2; Euchromatic histone-lysine N-methyltransferase 2; G 9a; G9 a; G9a protein; H3 K9 HMTase 3; H3 lysine-9 specific 3; H3-K9-HMTase 3; Histone H3 K9 methyltransferase 3; Histone H3 K9 methyltransferase3; Histone lysine N methyltransferase; Histone lysine N methyltransferase, H3 lysine 9 specific 3; Histone lysine N methyltransferase, H3 lysine 9 specific3; Histone-lysine N-methyltransferase; HLA B associated transcript 8; HLA-B associated transcript 8; KMT 1C; KMT1 C; Lysine N methyltransferase 1C; NG36; EHMT2_HUMAN; G9a; HLA-B-associated transcript 8; Histone H3-K9 methyltransferase 3; Protein G9a. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Rat (Human, Mouse, Dog, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human KMT1C/G9a |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 133 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Developmental Biology > Embryogenesis > Embryonic stem cells > Intracellular Developmental Biology > Reproduction > Germ cell markers Epigenetics and Nuclear Signaling > ChIP assays > ChIP antibodies Epigenetics and Nuclear Signaling > Chromatin Modifying Enzymes > Methylation Epigenetics and Nuclear Signaling > Chromatin Modifying Enzymes > Methylation > Lysine methylation Stem Cells > Embryonic Stem Cells > Intracellular Stem Cells > Germline Stem Cells > Embryonic Germ Cells |
| 亚基 | Heterodimer; heterodimerizes with EHMT1/GLP. Interacts with GFI1B and WIZ. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EHMT1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Part of a complex composed of TRIM28, HDAC1, HDAC2 and EHMT2. |
| 亚细胞定位 | Nucleus. Chromosome. Note=Associates with euchromatic regions. Does not associate with heterochromatin. |
| 组织特异性 | Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow. |
| 翻译后修饰 | Phosphorylated upon DNA damage, probably by ATM or ATR.
Methylated at Lys-185; automethylated. |
| 相似性 | Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 7 ANK repeats. Contains 1 post-SET domain. Contains 1 pre-SET domain. Contains 1 SET domain. |
| 功能 | Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | A cluster of genes, BAT1-BAT5, has been localized in the vicinity of the genes for TNF alpha and TNF beta. This gene is found near this cluster; it was mapped near the gene for C2 within a 120-kb region that included a HSP70 gene pair. These genes are all within the human major histocompatibility complex class III region. This gene was thought to be two different genes, NG36 and G9a, adjacent to each other but a recent publication shows that there is only a single gene. The protein encoded by this gene is thought to be involved in intracellular protein-protein interaction. There are three alternatively spliced transcript variants of this gene but only two are fully described. [provided by RefSeq, Jul 2008]. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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文献和实验该产品被引用文献
[IF={{ 4.4 }}] {Yesim Bilmez. et al.Histone lysine methyltransferases and their specific methylation marks show significant changes in mouse testes from young to older ages.BIOGERONTOLOGY.2025 Jan 20;26(1):42.} {IHC} {Mouse}
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KMT1C Rabbit pAb(bs-9118R)-50ul/100ul/200ul
￥1180 - 2800
