Sample: bone (Rat) Lysate at 40 ug
_x000D_
Primary: Anti- GPR84 (bs-13507R)at 1/300 dilution
_x000D_
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_
Predicted band size: 44kD
_x000D_
Observed band size: 44 kD
_x000D_
- ￥1180 - 2800
- Bioss
- bs-13507R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-13507R |
| 英文名称 | GPR84 Rabbit pAb |
| 中文名称 | G蛋白偶联受体84抗体 |
| 英文别名 | G protein coupled receptor 84; G-protein coupled receptor 84; G protein coupled receptor EX33; GPCR 4; GPCR4; GPR 84; GPR84_HUMAN; GPR84(EX33); EX33; Inflammation related G protein coupled receptor EX33; Inflammation-related G-protein coupled receptor EX33; GPCR EX33; EX 33; GPCR GPR84; |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Dog, Cow, Horse, Sheep, Monkey) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GPCR EX33/GPR84 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 44 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Signaling Pathway > G Protein Signaling > GPCR |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. |
| 组织特异性 | Expressed predominantly in hematopoietic tissues. High levels detected in the bone marrow and lower levels in the peripheral leukocytes and lung. Also expressed in brain, heart, muscle, colon, thymus, spleen, kidney, liver, placenta and intestine. Within the leukocyte population expression is higher in neutrophils and eosinophils relative to T- or B-lymphocytes. |
| 相似性 | Belongs to the G-protein coupled receptor 1 family. |
| 功能 | Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | G protein-coupled receptors (GPCRs), also designated seven transmembrane (7TM) receptors and heptahelical receptors, are a protein family which interact with G proteins (heterotrimeric GTPases) to synthesize intracellular second messengers such as diacylglycerol, cyclic AMP, inositol phosphates, and calcium ions. Their diverse biological functions range from vision and olfaction to neuronal and endocrine signaling and are involved in many pathological conditions. G protein receptor 84 (GPR84), a member of the GCPR 1 family, is an orphan GCPR expressed in bone marrow, brain, heart, muscle, colon, thymus, spleen, kidney, liver, placenta, intestine, lung and peripheral blood leukocytes. In activated T cells, GPR84 regulates early interleukin-4 (IL-4) gene expression |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
Sample:
_x000D_ SP2/0 (mouse)Cell Lysate at 40 ug
_x000D_ Lung(mouse) Lysate at 40 ug
_x000D_ HL-60 (human)Cell Lysate at 40 ug
_x000D_ Muscle (mouse) Lysate at 40 ug
_x000D_ Primary: Anti- GPR84 (bs-13507R)at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 44kD
_x000D_ Observed band size: 44 kD
_x000D_
_x000D_ SP2/0 (mouse)Cell Lysate at 40 ug
_x000D_ Lung(mouse) Lysate at 40 ug
_x000D_ HL-60 (human)Cell Lysate at 40 ug
_x000D_ Muscle (mouse) Lysate at 40 ug
_x000D_ Primary: Anti- GPR84 (bs-13507R)at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 44kD
_x000D_ Observed band size: 44 kD
_x000D_
Sample:
_x000D_ Lane 1: Blood cell (Mouse) Lysate at 40 ug
_x000D_ Lane 2: Lung (Mouse) Lysate at 40 ug
_x000D_ Lane 3: Bone (Rat) Lysate at 40 ug
_x000D_ Lane 4: Lung (Rat) Lysate at 40 ug
_x000D_ Lane 5: Cerebrum (Rat) Lysate at 40 ug
_x000D_ Lane 6: Cerebellum (Rat) Lysate at 40 ug
_x000D_ Lane 7: U937 (Human) Cell Lysate at 30 ug
_x000D_ Lane 8: U87MG (Human) Cell Lysate at 30 ug
_x000D_ Lane 9: THP-1 (Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti-GPR84 (bs-13507R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 44 kD
_x000D_ Observed band size: 44 kD
_x000D_ Lane 1: Blood cell (Mouse) Lysate at 40 ug
_x000D_ Lane 2: Lung (Mouse) Lysate at 40 ug
_x000D_ Lane 3: Bone (Rat) Lysate at 40 ug
_x000D_ Lane 4: Lung (Rat) Lysate at 40 ug
_x000D_ Lane 5: Cerebrum (Rat) Lysate at 40 ug
_x000D_ Lane 6: Cerebellum (Rat) Lysate at 40 ug
_x000D_ Lane 7: U937 (Human) Cell Lysate at 30 ug
_x000D_ Lane 8: U87MG (Human) Cell Lysate at 30 ug
_x000D_ Lane 9: THP-1 (Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti-GPR84 (bs-13507R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 44 kD
_x000D_ Observed band size: 44 kD
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GPR84 | EX33 Rabbit pAb(bs-13507R)-50ul/100ul/200ul
￥1180 - 2800
