Sample:
Lane 1: Human MOLT4 cell lysates
Lane 2: Human MCF-7 cell lysates
Lane 3: Human HeLa cell lysates
Lane 4: Human Jurkat cell lysates
Primary: Anti-MAML1 (bs-12396R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 108 kDa
Observed band size: 110 kDa
- ￥1180 - 2800
- Bioss
- bs-12396R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-12396R |
| 英文名称 | MAML1 Rabbit pAb |
| 中文名称 | 主导控制样蛋白1抗体 |
| 英文别名 | Mam-1; Mam1; MAML 1; MAML 1; Maml1; MAML1_HUMAN; Mastermind like 1; Mastermind-like protein 1; mKIAA0200. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Dog, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human MAML1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 108 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Stem Cells > Signaling Pathways > Notch > Nuclear |
| 亚基 | Interacts (via N-terminus) with NOTCH1, NOTCH2, NOTCH3 and NOTCH4 (via ankyrin repeat region). Interacts (via N-terminus) with p53 (via DNA-binding region). Forms a DNA-binding complex with Notch proteins and RBPSUH/RBP-J kappa/CBF1. Also binds CREBBP/CBP and CDK8. |
| 亚细胞定位 | Nucleus speckle. Nuclear, in a punctate manner. |
| 组织特异性 | Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen. |
| 相似性 | Belongs to the mastermind family. |
| 功能 | Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Notch receptors are involved in cell-fate determination in organisms as diverse as flies, frogs, and humans (1). The 'mastermind' gene has been identified in multiple genetic screens for modifiers of Notch mutations in Drosophila melanogaster (2). In Drosophila, loss-of-function mutations of Notch produce a 'neurogenic' phenotype in which cells destined to become epidermis switch fate and differentiate to neural cells (2). The human homolog, mastermind-like 1 (Mam1), localizes to nuclear bodies (2-4). Mam1 binds to the ankyrin repeat domain of all four mammalian Notch receptors, forms a DNA-binding complex with ICN and RBP-Jk, and amplifies Notch-induced transcription of Hes1 (2). Mam1 is an essential component of the transcriptional apparatus of Notch signaling (5). The gene which encodes Mam1 maps to human chromosome 5 (4). |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验该产品被引用文献
[IF={{ 3.063 }}] {Liu T et al. MicroRNA-193b-3p regulates hepatocyte apoptosis in selenium-deficient broilers by targeting MAML1.J Inorg Biochem. 2018 Sep;186:235-245.} {WB} {broiler chick}
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MAML1 Rabbit pAb(bs-12396R)-50ul/100ul/200ul
￥1180 - 2800
