Sample:
Skin (Mouse) Lysate at 40 ug
Skin (Rat) Lysate at 40 ug
Primary: Anti- Porimin (bs-7674R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kD
Observed band size: 64 kD
- ￥1180 - 2800
- Bioss
- bs-7674R
- 2025年10月16日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-7674R |
| 英文名称 | Porimin Rabbit pAb |
| 中文名称 | 前细胞胀亡受体诱导膜损伤蛋白抗体 |
| 英文别名 | KCT3; Keratinocytes associated transmembrane protein 3; Pro oncosis receptor inducing membrane injury; Serine/threonine rich receptor; TMEM123; Transmembrane protein 123; PORIM_HUMAN. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=2ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Dog, Pig, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Porimin |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 19 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 组织特异性 | Ubiquitous. Not expressed in ovary. Expressed in keratinocytes. |
| 相似性 | Belongs to the CD164 family. |
| 功能 | Implicated in oncotic cell death, characterized by cell swelling, organelle swelling, vacuolization and increased membrane permeability. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a highly glycosylated transmembrane protein with a high content of threonine and serine residues in its extracellular domain, similar to a broadly defined category of proteins termed mucins. Exposure of some cell types to anti PORIMIN (pro oncosis receptor inducing membrane injury) antibody, crosslinks this protein on the cell surface and induces a type of cell death termed oncosis. Oncosis is distinct from apoptosis and is characterized by a loss of cell membrane integrity without DNA fragmentation. This gene product is proposed to function as a cell surface receptor that mediates cell death. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {2ug/Test} |
Blank control:Molt4.
Primary Antibody (green line): Rabbit Anti-Porimin antibody (bs-7674R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-Porimin antibody (bs-7674R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Sample:
Embryo (Mouse) Lysate at 40 ug
Primary: Anti- Porimin (bs-7674R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kD
Observed band size: 74 kD
Embryo (Mouse) Lysate at 40 ug
Primary: Anti- Porimin (bs-7674R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kD
Observed band size: 74 kD
Sample:
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti- Porimin (bs-7674R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kD
Observed band size: 74 kD
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti- Porimin (bs-7674R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19 kD
Observed band size: 74 kD
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
[IF={{ 5.6 }}] {Dongjie Zhang. et al. Dihydrotanshinone Triggers Porimin-Dependent Oncosis by ROS-Mediated Mitochondrial Dysfunction in Non-Small-Cell Lung Cancer. INT J MOL SCI. 2023 Jan;24(15):11953} {WB} {Mouse}
[IF={{ 3.69 }}] {Sukfan P. Kwong. et al. PORIMIN: The key to (+)-Usnic acid-induced liver toxicity and oncotic cell death in normal human L02 liver cells. J Ethnopharmacol. 2021 Apr;270:113873} {WB} {Human}
[IF={{ 2.9 }}] {Chengcheng Li. et al.Oncosis is the predominant type of cell death in rhabdomyolysis following exertional heat stroke..PLoS One.2025 Mar 25;20(3):e0308586.} {Western blot,IHC,Flow cytometry} {Rat,Human}
技术资料暂无技术资料 索取技术资料
文献支持
Porimin Rabbit pAb(bs-7674R)-50ul/100ul/200ul
￥1180 - 2800
