Protein:spleen(mouse) lysates at 40ug;
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Primary: Anti-Il-12 beta (bs-10641R) at 1:300;
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Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000;
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ECL excitated the fluorescence;
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Predicted band size : 35 kD
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Observed band size : 37 kD
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- ￥1180 - 2800
- Bioss
- bs-10641R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-10641R |
| 英文名称 | IL12B Rabbit pAb |
| 中文名称 | 白细胞介素12 β亚基蛋白抗体 |
| 英文别名 | CLMF; CLMF p40; CLMF2; Cytotoxic lymphocyte maturation factor 40 kDa subunit; IL 12 subunit p40; IL 12B; IL-12 subunit p40; IL-12B; IL12 subunit p40; IL12 p40; IL12B_HUMAN; interleukin 12 beta chain; Interleukin 12 p40; Interleukin 12 subunit beta; Interleukin 12B; Interleukin-12 subunit beta; natural killer cell stimulatory factor 40 kD subunit; NK cell stimulatory factor chain 2; NKSF; NKSF2; p40. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Pig, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human IL12B |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 35 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-IL-12 beta Polyclonal Antibody, Unconjugated(bs-10641R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-IL-12 beta Polyclonal Antibody, Unconjugated(bs-10641R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
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文献和实验该产品被引用文献
[IF=4.2] Dan-Qi Xu. et al.To explore the potential combined treatment strategy for colorectal cancer: Inhibition of cancer stem cells and enhancement of intestinal immune microenvironment..European Journal of Pharmacology.2025 Mar 20:998:177533. Western blot Mouse
[IF=1.48] Li, Kang, et al. "CD14 knockdown reduces lipopolysaccharide-induced cell viability and expression of inflammation-associated genes in gastric cancer cells in vitro and in nude mouse xenografts." Molecular Medicine Reports. Other =""
[IF=1.27] Sun, Zhenqiang, et al. "Immunological effect induced by mesenchymal stem cells in a rat liver transplantation model." Experimental and therapeutic medicine 10.2 (2015): 401-406. IHC ="Rat"
相关实验
, Algermissen, B. et al. 3. Purification of rabbit liver aldehyde oxidase by affinity chromatography on benzamidine Sepharose 6B. J. Chromatogr. (1989), 475 363-72, Stell, J. G. P. et al. 4. The Crystal Structure of a T Cell Receptor in Complex
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
. 7:8‐16. Christie, M.R., Hollands, J.A., Brown, T.J., Michelsen, B.K., and Delovitch, T.L. 1993. Detection of pancreatic islet 64,000 M(r) autoantigens in insulin‐dependent
Models and Analysis of Atherosclerosis, Restenosis, and Aneurysm Formation in the Mouse
Avidin‐biotin complex (ABC) kit (Dako) 3,3′‐diaminobenzidine (Bright DAB solution; ImmunoLogic, cat. no. BS04‐500A/B; http://www.immunologic.nl
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IL12B | IL-12B Rabbit pAb(bs-10641R)-50ul/100ul/200ul
￥1180 - 2800
