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ADIPOR2/PAQR2 Rabbit pAb(bs-06

11R)-50ul/100ul/200ul
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  • ￥1180 - 2800
  • Bioss已认证
  • bs-0611R
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-0611R
    英文名称ADIPOR2/PAQR2 Rabbit pAb
    中文名称脂联素受体2抗体
    英文别名PAQR2_HUMAN; Adiponectin receptor protein 2; Progestin and adipoQ receptor family member 2 ; Progestin and adipoQ receptor family member II; ACDCR2;
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat (Chicken, Dog, Pig, Cow)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human Adiponectin receptor 2
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量44 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cardiovascular > Atherosclerosis > Diabetes associated

    Cardiovascular > Atherosclerosis > Lipoprotein metabolism

    Cardiovascular > Lipids / Lipoproteins > Fatty Acids > Metabolism

    Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Energy Metabolism

    Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Fatty acids

    Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Lipoprotein metabolism

    Metabolism > Pathways and Processes > Redox metabolism > Fatty acid oxidation

    Metabolism > Types of disease > Cancer

    Metabolism > Types of disease > Diabetes

    Metabolism > Types of disease > Heart disease

    Signal Transduction > Growth Factors/Hormones > Hormones

    Signal Transduction > Metabolism > Energy Metabolism

    Signal Transduction > Metabolism > Lipid metabolism

    亚基May form homo and heteromultimers.
    亚细胞定位Membrane; Multi-pass membrane protein. Note=Localized to the cell membrane and intracellular organelles.
    组织特异性Highly expressed in skeletal muscle, liver and placenta. Weakly expressed in brain, heart, colon, spleen, kidney, thymus, small intestine, peripheral blood leukocytes and lung.
    相似性Belongs to the ADIPOR family.
    功能Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some intermediate-affinity receptor activity for both globular and full-length adiponectin.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The adiponectin receptors, ADIPOR1 (MIM 607945) and ADIPOR2, serve as receptors for globular and full-length adiponectin (MIM 605441) and mediate increased AMPK (see MIM 602739) and PPAR-alpha (PPARA; MIM 170998) ligand activities, as well as fatty acid oxidation and glucose uptake by adiponectin (Yamauchi et al., 2003 [PubMed 12802337]).[supplied by OMIM, Mar 2008]

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}
    {Flow-Cyt}{1μg /test}

     

    产品细节图片1
    Tissue/cell: rat pancreas tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated(bs-0611R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    产品细节图片2
    Tissue/cell: human rectal tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated(bs-0611R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    产品细节图片3
    Blank control: HepG2(blue).
    Primary Antibody:Rabbit Anti-Adiponectin receptor 2 antibody (bs-0611R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
    Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;
    Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
    Protocol
    The cells were fixed with 2% P‌‌araformaldehyde for 10 min at 37℃. Primary antibody (bs-0611R, 0.2μg /1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Acquisition of 20,000 events was performed.
    产品细节图片4
    Sample:
    Lane 1: Heart (Mouse) Lysate at 40 ug
    Lane 2: Skeletal muscle (Mouse) Lysate at 40 ug
    Lane 3: Heart (Rat) Lysate at 40 ug
    Lane 4: Skeletal muscle (Rat) Lysate at 40 ug
    Lane 5: 293T (Human) Cell Lysate at 30 ug
    Primary: Anti-Adiponectin receptor 2 (bs-0611R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 44 kD
    Observed band size: 43 kD
    产品细节图片5
    P‌‌araformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Adiponectin receptor 2) Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片6
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片7
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片8
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片9
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片10
    P‌‌araformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片11
    P‌‌araformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片12
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片13
    P‌‌araformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片14
    P‌‌araformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片15
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片16
    P‌‌araformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片17
    P‌‌araformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片18
    P‌‌araformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

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    该产品被引用文献

    [IF={{ 6.43 }}] {Morad, Vivian, Annelie Abrahamsson, and Charlotta Dabrosin. "Estradiol affects extracellular leptin: adiponectin ratio in human breast tissue in vivo." The Journal of Clinical Endocrinology & Metabolism (2014).} {IHC} {="Human"}

    [IF={{ 5.1 }}] {Zacharias Amanda M.. et al. Site- and cell-type-specific miRNA and mRNA genes and networks across the cortex, striatum, and hypothalamus. COMMUN BIOL. 2025 Jul;8(1):1-15} {IF} {Mouse}

    [IF={{ 5.037 }}] {Annamária Schaffer. et al. The ontogenies of endometrial and myometrial leptin and adiponectin receptors in pregnant rats: Their putative impact on uterine contractility. Life Sci. 2022 May;297:120465} {WB} {Rat}

    [IF={{ 4.75 }}] {Guo et al. Effect of telmisartan on the expression of adiponectin receptors and nicotinamide adenine dinucleotide phosphate oxidase in the heart and aorta in type 2 diabetic rats. (2012) Cardiovasc.Diabetol. 11:94} {IHC} {Rat}

    [IF={{ 4.4 }}] {Jerad H. Dumolt. et al. Trophoblast-specific overexpression of adiponectin receptor 2 causes fetal growth restriction in pregnant mice. FASEB J. 2024 Oct;38(19):e70100} {WB} {Mouse}

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