Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_
Incubation: Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated(bs-0611R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
_x000D_
- ￥1180 - 2800
- Bioss
- bs-0611R
- 2026年10月13日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
细胞污染识别与处理全攻略：5 种常见类型+关键误区Cytoscape 教程来了！快速实现顶刊同款通路网络图五大应用案例：Mustang Q 膜层析应用全解析1 个小工具，一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0611R |
| 英文名称 | ADIPOR2/PAQR2 Rabbit pAb |
| 中文名称 | 脂联素受体2抗体 |
| 英文别名 | PAQR2_HUMAN; Adiponectin receptor protein 2; Progestin and adipoQ receptor family member 2 ; Progestin and adipoQ receptor family member II; ACDCR2; |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Chicken, Dog, Pig, Cow) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Adiponectin receptor 2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 44 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Atherosclerosis > Diabetes associated Cardiovascular > Atherosclerosis > Lipoprotein metabolism Cardiovascular > Lipids / Lipoproteins > Fatty Acids > Metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Energy Metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Fatty acids Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Lipoprotein metabolism Metabolism > Pathways and Processes > Redox metabolism > Fatty acid oxidation Metabolism > Types of disease > Cancer Metabolism > Types of disease > Diabetes Metabolism > Types of disease > Heart disease Signal Transduction > Growth Factors/Hormones > Hormones Signal Transduction > Metabolism > Energy Metabolism Signal Transduction > Metabolism > Lipid metabolism |
| 亚基 | May form homo and heteromultimers. |
| 亚细胞定位 | Membrane; Multi-pass membrane protein. Note=Localized to the cell membrane and intracellular organelles. |
| 组织特异性 | Highly expressed in skeletal muscle, liver and placenta. Weakly expressed in brain, heart, colon, spleen, kidney, thymus, small intestine, peripheral blood leukocytes and lung. |
| 相似性 | Belongs to the ADIPOR family. |
| 功能 | Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some intermediate-affinity receptor activity for both globular and full-length adiponectin. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The adiponectin receptors, ADIPOR1 (MIM 607945) and ADIPOR2, serve as receptors for globular and full-length adiponectin (MIM 605441) and mediate increased AMPK (see MIM 602739) and PPAR-alpha (PPARA; MIM 170998) ligand activities, as well as fatty acid oxidation and glucose uptake by adiponectin (Yamauchi et al., 2003 [PubMed 12802337]).[supplied by OMIM, Mar 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1μg /test} |
Tissue/cell: human rectal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated(bs-0611R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_ _x000D_
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-Adiponectin Receptor 2 Polyclonal Antibody, Unconjugated(bs-0611R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_ _x000D_
Blank control: HepG2(blue).
Primary Antibody:Rabbit Anti-Adiponectin receptor 2 antibody (bs-0611R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
_x000D_ _x000D_ Protocol
_x000D_ The cells were fixed with 2% Paraformaldehyde for 10 min at 37℃. Primary antibody (bs-0611R, 0.2μg /1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
Primary Antibody:Rabbit Anti-Adiponectin receptor 2 antibody (bs-0611R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
_x000D_ _x000D_ Protocol
_x000D_ The cells were fixed with 2% Paraformaldehyde for 10 min at 37℃. Primary antibody (bs-0611R, 0.2μg /1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
Sample:
_x000D_ Lane 1: Heart (Mouse) Lysate at 40 ug
_x000D_ Lane 2: Skeletal muscle (Mouse) Lysate at 40 ug
_x000D_ Lane 3: Heart (Rat) Lysate at 40 ug
_x000D_ Lane 4: Skeletal muscle (Rat) Lysate at 40 ug
_x000D_ Lane 5: 293T (Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti-Adiponectin receptor 2 (bs-0611R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 44 kD
_x000D_ Observed band size: 43 kD
_x000D_ Lane 1: Heart (Mouse) Lysate at 40 ug
_x000D_ Lane 2: Skeletal muscle (Mouse) Lysate at 40 ug
_x000D_ Lane 3: Heart (Rat) Lysate at 40 ug
_x000D_ Lane 4: Skeletal muscle (Rat) Lysate at 40 ug
_x000D_ Lane 5: 293T (Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti-Adiponectin receptor 2 (bs-0611R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 44 kD
_x000D_ Observed band size: 43 kD
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Adiponectin receptor 2) Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Adiponectin receptor 2 Polyclonal Antibody, Unconjugated (bs-0611R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
询价记录
暂无询价记录
文献和实验
技术资料暂无技术资料 索取技术资料
文献支持
ADIPOR2 | PAQR2 Rabbit pAb(bs-0611R)-50ul/100ul/200ul
￥1180 - 2800
