Sample:
_x000D_
JAR(Human) Cell Lysate at 30 ug
_x000D_
MCF-7(Human) Cell Lysate at 30 ug
_x000D_
Primary: Anti- CD276 (bs-11019R) at 1/1000 dilution
_x000D_
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_
Predicted band size: 54 kD
_x000D_
Observed band size: 54 kD
_x000D_
- ￥1180 - 2800
- Bioss
- bs-11019R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-11019R |
| 英文名称 | B7-H3/CD276 Rabbit pAb |
| 中文名称 | CD276抗体 |
| 英文别名 | B7H3; 4Ig B7 H3; 4Ig-B7-H3; AU016588; B7 H3; B7 homolog 3; B7H3; B7RP-2; CD_antigen=CD276; CD276 antigen; CD276; Costimulatory molecule; PSEC0249; UNQ309/PRO352; CD276_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Pig, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CD276/B7H3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 54 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Tumor biomarkers Immunology > Adaptive Immunity > T Cells > CD Immunology > Immune Interventions > Transplantation Stem Cells > Hematopoietic Progenitors > Lymphoid > T Lymphocytic Lineage |
| 亚基 | Interacts with TREML2 and this interaction enhances T-cell activation. |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein (Probable). |
| 组织特异性 | Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta. |
| 相似性 | Belongs to the immunoglobulin superfamily. BTN/MOG family. _x000D_ Contains 2 Ig-like C2-type (immunoglobulin-like) domains. _x000D_ Contains 2 Ig-like V-type (immunoglobulin-like) domains. |
| 功能 | May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: Human laryngeal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-CD276 Polyclonal Antibody, Unconjugated(bs-11019R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-CD276 Polyclonal Antibody, Unconjugated(bs-11019R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
Sample:
_x000D_ U-2OS(Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti- CD276 (bs-11019R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 54 kD
_x000D_ Observed band size: 54 kD
_x000D_
_x000D_ U-2OS(Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti- CD276 (bs-11019R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 54 kD
_x000D_ Observed band size: 54 kD
_x000D_
Sample:
_x000D_ 293T(Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti- CD276 (bs-11019R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 54 kD
_x000D_ Observed band size: 54 kD
_x000D_
_x000D_ 293T(Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti- CD276 (bs-11019R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 54 kD
_x000D_ Observed band size: 54 kD
_x000D_
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文献和实验该产品被引用文献
[IF=4.96] Nan Cheng. et al. B7-H3 augments the pro-angiogenic function of tumor-associated macrophages and acts as a novel adjuvant target for triple-negative breast cancer therapy. Biochem Pharmacol. 2021 Jan;183:114298 IF Mouse
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CD276 | B7-H3 Rabbit pAb(bs-11019R)-50ul/100ul/200ul
￥1180 - 2800
