Blank control (blue line): Hela (blue).
Primary Antibody (green line): Rabbit Anti-MDR1 antibody (bs-1468R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% methanol (Overnight at -20℃) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-1468R
- 2025年10月16日
- 产品信息以Bioss网站为准
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1468R |
| 英文名称 | MDR1/P Glycoprotein Rabbit pAb |
| 中文名称 | 多药耐药蛋白/P-糖蛋白(C端)抗体 |
| 英文别名 | ABC20; ABCB1; ATP binding cassette, sub family B(MDR/TAP), member 1; ATP-binding cassette sub-family B member 1; CD243; CLCS; Colchicin sensitivity; Doxorubicin resistance; GP170; MDR1; MDR1_HUMAN; Multidrug resistance 1; Multidrug resistance protein 1; P glycoprotein 1; P gp; P-glycoprotein 1; PGY1. |
| 中文别名 | p-gp; P糖蛋白 |
| 产品应用 | WB=1:500-2000, ICC/IF=1:100-500, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human MDR1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 141 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Drug resistance > P glycoproteins Signal Transduction > Metabolism > Plasma Membrane > ATPases Signal Transduction > Metabolism > Plasma Membrane > Channels Stem Cells > Hematopoietic Progenitors > Side Population Stem Cells > Hematopoietic Progenitors > Surface Molecules |
| 亚基 | Interacts with PSMB5. |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. |
| 组织特异性 | Expressed in liver, kidney, small intestine and brain. |
| 相似性 | Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains. |
| 功能 | Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. Subunit : Interacts with PSMB5. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | bs-1468P is one synthetic peptide derived from human MDR1. P Glycoprotein, the product of the MDR1 gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of P Glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P Glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P Glycoprotein have also been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias / lymphomas. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {ICC/IF} | {1:100-500} |
| {Flow-Cyt} | {1μg/Test} |
Sample: liver (Rat) Lysate at 40 ug
Primary: Anti-MDR1'P Glycoprotein (bs-1468R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 141kD
Observed band size: 141 kD
Primary: Anti-MDR1'P Glycoprotein (bs-1468R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 141kD
Observed band size: 141 kD
Tissue/cell:SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (MDR1/P Glycoprotein) polyclonal Antibody, Unconjugated (bs-1468R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell:SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (MDR1/P Glycoprotein) polyclonal Antibody, Unconjugated (bs-1468R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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文献和实验该产品被引用文献
[IF={{ 6.656 }}] {Jiameng Qu. et al. Induction of P-glycoprotein expression by dandelion in tumor and heart tissues: Impact on the anti-tumor activity and cardiotoxicity of doxorubicin. PHYTOMEDICINE. 2022 Jun;:154275} {WB} {Mouse}
[IF={{ 5.988 }}] {Zipeng Gong. et al. Pharmacokinetic Differences of Wuji Pill Components in Normal and Chronic Visceral Hypersensitivity Irritable Bowel Syndrome Rats Attributable to Changes in Tight Junction and Transporters. FRONT PHARMACOL. 2022; 13: 948678} {IHC,IF} {Rat}
[IF={{ 4.546 }}] {Huang, Wenjie. et al. The inhibitory effect and mechanism of Yi-qi-hua-yu-jie-du decoction on the drug resistance of gastric cancer stem cells based on ABC transporters. CHIN MED-UK. 2022 Dec;17(1):1-18} {WB} {Human}
[IF={{ 3.448 }}] {Zhang E et al. 7-O-geranylquercetin contributes to reverse P-gp-mediated adriamycin resistance in breast cancer. Life Sci. 2019 Oct 5:116938.} {WB,IHC} {Human}
[IF={{ 3.407 }}] {Du J et al. MAY, a novel tubulin inhibitor, induces cell apoptosis in A549 and A549/Taxol cells and inhibits epithelial-mesenchymal transition in A549/Taxol cells. Chem Biol Interact. 2020 May 25;323:109074.} {WB} {human}
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MDR1/P Glycoprotein Rabbit pAb(bs-1468R)-50ul/100ul/200ul
￥1180 - 2800
