Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Sulfatase 2 Polyclonal Antibody, Unconjugated(bs-3894R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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- 详细信息
- 技术资料
- 应用范围:
产品信息以Bioss网站为准
- 规格:
50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-3894R |
| 英文名称 | Sulfatase 2 Rabbit pAb |
| 中文名称 | 硫酸酯酶2蛋白抗体 |
| 英文别名 | Extracellular sulfatase Sulf 2; HSulf2; SULF2; SULF-2; SULF 2. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Rat (Human, Mouse, Chicken, Dog, Pig, Cow, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Sulfatase 2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 93 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Cellular metabolic process Metabolism > Pathways and Processes > Metabolic signaling pathways > Amino acid metabolism Metabolism > Types of disease > Cancer Signal Transduction > Cytoskeleton / ECM > HSPGs Signal Transduction > Metabolism > Amino Acids |
| 亚细胞定位 | Endoplasmic reticulum. Golgi apparatus, Golgi stack. Cell surface. Note=Also localized on the cell surface. |
| 组织特异性 | Expressed at highest levels in the ovary, skeletal muscle, stomach, brain, uterus, heart, kidney and placenta. |
| 翻译后修饰 | The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. |
| 功能 | Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Sulfatase 2 exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. It is expressed at highest levels in the ovary, skeletal muscle, stomach, brain, uterus, heart, kidney and placenta. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |

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Sulfatase 2 Rabbit pAb(bs-3894R)-50ul/100ul/200ul
¥1180 - 2800





