Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ACTH(7-23) Polyclonal Antibody, Unconjugated(bs-0004R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-0004R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0004R |
| 英文名称 | ACTH (7-23) Rabbit pAb |
| 中文名称 | 促肾上腺皮质激素ACTH (7-23)抗体 |
| 英文别名 | Adrenocorticotropic hormone; Adrenocorticotropin; Adrenocorticotropin Hormone; alpha Melanocyte Stimulating Hormone; beta Endorphin; beta Lipotropin; beta Melanocyte Stimulating Hormone; CLIP; Corticotropin; Corticotropin Like Intermediary Peptide; Corticotropin lipotropin precursor; Lipotropin beta; Lipotropin gamma; LPH; Melanotropin alpha; Met Enkephalin; MSH; NPP; POC; POMC; Pro opiomelanocortin; Proopiomelanocortin. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Dog, Pig, Cow, Rabbit, GuineaPig, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human ACTH |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 4.3 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Hormone biosynthesis Metabolism > Pathways and Processes > Endocrine metabolism > Hormone biosynthesis Neuroscience > Endocrine system > Hypothalamic pituitary adrenal axis Neuroscience > Neurotransmitter > Neuropeptides > Hormones Signal Transduction > Growth Factors/Hormones > Hormones |
| 亚基 | Belongs to the POMC family. |
| 亚细胞定位 | Secreted. |
| 组织特异性 | ACTH and MSH are produced by the pituitary gland. |
| 翻译后修饰 | Specific enzymatic cleavages at paired basic residues yield the different active peptides.
O-glycosylated; reducing sugar is probably N-acetylgalactosamine. |
| 相似性 | Belongs to the POMC family. |
| 功能 | ACTH stimulates the adrenal glands to release cortisol.
MSH (melanocyte-stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin and Met-enkephalin are endogenous opiates. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
Lane 1: Pituitary (Mouse) Tissue Lysate
Lane 2: Pituitary (Rat) Tissue Lysate
Primary:
Anti-ACTH (7-23) (bs-0004R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 4.3 kD
Observed band size: 31/22/13 kD
Lane 1: Pituitary (Mouse) Tissue Lysate
Lane 2: Pituitary (Rat) Tissue Lysate
Primary:
Anti-ACTH (7-23) (bs-0004R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 4.3 kD
Observed band size: 31/22/13 kD
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文献和实验该产品被引用文献
[IF={{ 7.081 }}] {Qingxing Liu. et al. Head-to-head comparison of 68Ga-DOTATATE PET/CT and 18F-FDG PET/CT in localizing tumors with ectopic adrenocorticotropic hormone secretion: a prospective study. 2021 Jun 19} {IHC} {Human}
[IF={{ 4.94 }}] {Zhou, Ding'an, et al. "A novel P53/POMC/Gαs/SASH1 autoregulatory feedback loop activates mutated SASH1 to cause pathologic hyperpigmentation." Journal of Cellular and Molecular Medicine (2016).} {IHC} {="Human"}
[IF={{ 2.96 }}] {Jussila, Anna, et al. "Narrow-band ultraviolet B radiation induces the expression of β-endorphin in human skin in vivo." Journal of Photochemistry and Photobiology B: Biology (2016).} {IHC} {="Human"}
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ACTH (7-23) Rabbit pAb(bs-0004R)-50ul/100ul/200ul
￥1180 - 2800
