Sample:K562 Cell (Human) Lysate at 40 ug
Primary: Anti-CTH (bs-9515R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 45 kD
- ￥1180 - 2800
- Bioss
- bs-9515R
- 2025年10月24日
- 产品信息以Bioss网站为准
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-9515R |
| 英文名称 | CTH Rabbit pAb |
| 中文名称 | 胱硫醚γ裂解酶抗体 |
| 英文别名 | CGL_HUMAN; CTH; Cystathionine gamma lyase; Cystathionine gamma-lyase; Cysteine desulfhydrase; gamma cystathionase; gamma-cystathionase; Homoserine deaminase; Homoserine dehydratase; MGC9471; CSE; Cystathionase. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=2ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Dog, Cow, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CTH/CSE/Cystathionase |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 45 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Metabolism > Pathways and Processes > Metabolic signaling pathways > Amino acid metabolism Signal Transduction > Metabolism > Amino Acids |
| 亚基 | Homotetramer. Interacts with CALM in a calcium-dependent manner. |
| 亚细胞定位 | Cytoplasm |
| 翻译后修饰 | Phosphorylated upon DNA damage, probably by ATM or ATR. |
| 相似性 | Belongs to the trans-sulfuration enzymes family. |
| 功能 | Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into –SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Catalyzes the last step in the transsulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure.Defects in CTH are the cause of cystathioninuria (CSTNU). It is an autosomal recessive phenotype characterized by abnormal accumulation of plasma cystathionine, leading to increased urinary excretion. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {2ug/Test} |
Sample:Esophagus (Mouse) Lysate at 40 ug
Primary: Anti-CTH (bs-9515R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 45 kD
Primary: Anti-CTH (bs-9515R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 45 kD
Sample:Liver (Mouse) Lysate at 40 ug
Primary: Anti-CTH (bs-9515R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 48 kD
Primary: Anti-CTH (bs-9515R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 48 kD
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-CTH antibody (bs-9515R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-CTH antibody (bs-9515R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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文献和实验该产品被引用文献
[IF={{ 9.8 }}] {Xiaoyan Zheng. et al. Novel findings from arsenic‑lead combined exposure in mouse testicular TM4 Sertoli cells based on transcriptomics. SCI TOTAL ENVIRON. 2023 Dec;:169611} {WB} {Mouse}
[IF={{ 5.81 }}] {Heng-Jing Hu. et al. Hydrogen Sulfide Ameliorates Angiotensin II-Induced Atrial Fibrosis Progression to Atrial Fibrillation Through Inhibition of the Warburg Effect and Endoplasmic Reticulum Stress. Front Pharmacol. 2021 Dec 7;12:690371} {WB} {Mouse,Rat}
[IF={{ 4.9 }}] {Bao Yuan. et al. Exploring the Mechanism of H2S Synthesis in Male Bactrian Camel Poll Glands Based on Data Independent Acquisition Proteomics and Non-Targeted Metabolomics. INT J MOL SCI. 2024 Jul; 25(14): 7700} {WB,IHC,IF} {Camel}
[IF={{ 4.8 }}] {Qiang Ran. et al. Integrated bioinformatics and multi-omics to investigate the mechanism of Rhododendron molle Flos-induced hepatotoxicity. J ETHNOPHARMACOL. 2024 Dec;:119308} {WB} {Rat}
[IF={{ 4.8 }}] {Mengjun Huang. et al.Integrated bioinformatics and multi-omics to investigate the mechanism of Rhododendron molle Flos-induced hepatotoxicity.journal of ethnopharmacology.2025 Feb 11:341:119308.} {Western Blot} {Rat}
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CTH Rabbit pAb(bs-9515R)-50ul/100ul/200ul
￥1180 - 2800
