Blank control:U937.
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Primary Antibody (green line): Rabbit Anti-CNTNAP4 antibody (bs-11076R)
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Dilution: 2μg /10^6 cells;
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Isotype Control Antibody (orange line): Rabbit IgG .
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Secondary Antibody : Goat anti-rabbit IgG-PE
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Dilution: 1μg /test.
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Protocol
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The cells wereincubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
- ￥1180 - 2800
- Bioss
- bs-11076R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-11076R |
| 英文名称 | CNTNAP4 Rabbit pAb |
| 中文名称 | 接触蛋白相关蛋白4抗体 |
| 英文别名 | CASPR 4; CASPR4; Cell recognition molecule Caspr4; Cell recognition protein CASPR4; CNTNAP 4; CNTP4_HUMAN; Contactin associated protein like 4; Contactin-associated protein-like 4. |
| 产品应用 | Flow-Cyt=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Dog, Pig, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CNTNAP4 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 143 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Cell Adhesion Proteins > Membrane Proteins |
| 亚细胞定位 | Membrane. |
| 相似性 | Belongs to the neurexin family. Contains 2 EGF-like domains. Contains 1 F5/8 type C domain. Contains 1 fibrinogen C-terminal domain. Contains 4 laminin G-like domains. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Neurexins comprise a family of neuronal cell surface proteins, which include neurexin I (NRXN1), neurexin II (NRXN2), neurexin III (NRXN3) and CASPR (neurexin IV). CASPR4 (contactin associated protein-like 4), also known as CNTNAP4, is a 1,308 amino acid single-pass type I membrane protein belonging to the neurexin family. Possibly a cell adhesion protein, CASPR4 is expressed in multiple regions of the brain such as olfactory bulb, hippocampus and deep cerebellar nuclei, with highest expression found in spinal cord, substantia nigra and subthalamic nucleus. CASPR4 is expressed at moderate levels in fetal brain, with low expression in adult testis. Encoded by a gene that maps to human chromosome 16q23.1, CASPR4 contains four laminin G-like domains, a F5/8 type C domain, two EGF-like domains and a single fibrinogen C-terminal domain. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1:100-500} |
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文献和实验该产品被引用文献
[IF=8.063] Zhang W et al. CNTNAP4 deficiency in dopaminergic neurons initiates parkinsonian phenotypes. Theranostics. 2020 Feb 10;10(7):3000-3021. ICC mouse
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CNTNAP4 | CASPR4 Rabbit pAb(bs-11076R)-50ul/100ul/200ul
￥1180 - 2800
