Sample: U937 Cell Lysate at 30 ug
Primary: Anti- CD69 (bs-2499R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 34 kD
- ￥1180 - 2800
- Bioss
- bs-2499R
- 2025年10月16日
- 产品信息以Bioss网站为准
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2499R |
| 英文名称 | CD69 Rabbit pAb |
| 中文名称 | 活化诱导分子CD69抗体 |
| 英文别名 | CLEC2C; AIM; Early activation antigen CD69; Early T-cell activation antigen p60; GP32/28; Leukocyte surface antigen Leu-23; MLR-3; EA1; BL-AC/P26; C-type lectin domain family 2 member C; CD69_HUMAN; |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Dog) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CD69 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 22 kDa |
| 实际分子量 | 27-33 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Immunology > Adaptive Immunity > Regulatory T Cells Immunology > Adaptive Immunity > T Cells > CD Immunology > Cell Type Markers > CD > NK Cells Stem Cells > Hematopoietic Progenitors > Lymphoid > B Lymphocytic Lineage Stem Cells > Hematopoietic Progenitors > Lymphoid > NK Cell Lineage Stem Cells > Hematopoietic Progenitors > Lymphoid > T Lymphocytic Lineage |
| 亚基 | Homodimer; disulfide-linked. |
| 亚细胞定位 | Membrane; Single-pass type II membrane protein. |
| 组织特异性 | Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets. |
| 翻译后修饰 | Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes. |
| 相似性 | Contains 1 C-type lectin domain. |
| 功能 | Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. [provided by RefSeq, Aug 2011] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {1μg/Test} |
Blank control(blue): Molt-4 Cells(fixed with 2% Paraformaldehyde (10 min)) .
Primary Antibody: Rabbit Anti-CD69/FITC Conjugated antibody (bs-2499R/FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
Primary Antibody: Rabbit Anti-CD69/FITC Conjugated antibody (bs-2499R/FITC), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
Sample:
Lane 1: Blood cell (Mouse) Lysate at 40 ug
Lane 2: Spleen (Mouse) Lysate at 40 ug
Primary: Anti-CD69 (bs-2499R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33/27 kD
Observed band size: 30 kD
Lane 1: Blood cell (Mouse) Lysate at 40 ug
Lane 2: Spleen (Mouse) Lysate at 40 ug
Primary: Anti-CD69 (bs-2499R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33/27 kD
Observed band size: 30 kD
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文献和实验该产品被引用文献
[IF={{ 8.8 }}] {Hashimoto Masashi. et al. Long-term activation of anti-tumor immunity in pancreatic cancer by a p53-expressing telomerase-specific oncolytic adenovirus. BRIT J CANCER. 2024 Feb;:1-9} {IHC} {Human}
[IF={{ 4.12 }}] {Mokuda et al. CD1a+ survivin+ dendritic cell infiltration in dermal lesions of systemic sclerosis. (2015) Arthritis.Res.Ther. 17:275} {IHC} {Human}
[IF={{ 14.6 }}] {KeLun Zhang. et al. Peripherally induced RORγt+ skin-resident regulatory T cells mediate the efficacy of allergen-specific immunotherapy. SCI TRANSL MED. 2025 May;17(800)} {IF} {Mouse,Human}
[IF={{ 12.121 }}] {Rouven Hoefflin. et al. HIF-1α and HIF-2α differently regulate tumour development and inflammation of clear cell renal cell carcinoma in mice. Nat Commun. 2020 Aug;11(1):1-21} {IHC} {Mouse}
[IF={{ 10.711 }}] {Zihuan Luo. et al. Ex vivo anchored PD-L1 functionally prevent in vivo renal allograft rejection. BIOENG TRANSL MED. 2022 Apr 06} {FCM} {Rat}
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CD69 Rabbit pAb(bs-2499R)-50ul/100ul/200ul
￥1180 - 2800
