Blank control (Black line): Molt4 (Black).
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Primary Antibody (green line):Rabbit Anti-KLLN antibody (bs-6893R)
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Dilution:1μg /10^6 cells;
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Isotype Control Antibody (orange line): Rabbit IgG .
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Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
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Dilution: 1μg /test.
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Protocol
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The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-6893R
- 2026年10月13日
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- 技术资料
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-6893R |
| 英文名称 | KLLN Rabbit pAb |
| 中文名称 | Killin-p53调节复制抑制蛋白抗体 |
| 英文别名 | KILIN_HUMAN; Killin; killin, p53-regulated DNA replication inhibitor; KLLN. |
| 产品应用 | Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human KLLN |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 20 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Apoptosis > Intracellular > p53 Pathway Cell Biology > Cell Cycle > Cell Cycle Inhibitors > p53 Epigenetics and Nuclear Signaling > Chromatin Binding Proteins > DNA / RNA binding Epigenetics and Nuclear Signaling > DNA / RNA > DNA Synthesis |
| 亚细胞定位 | Nucleus. |
| 功能 | DNA-binding protein involved in S phase checkpoint control-coupled apoptosis by mediating p53/TP53-induced apoptosis. Has the ability to inhibit DNA synthesis and S phase arrest coupled to apoptosis. Has affinity to both double- and single-stranded DNA. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | DNA-binding protein involved in S phase checkpoint control-coupled apoptosis by mediating p53/TP53-induced apoptosis. Has the ability to inhibit DNA synthesis and S phase arrest coupled to apoptosis. Has affinity to both double- and single-stranded DNA. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1ug/Test} |
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KLLN | Killin Rabbit pAb(bs-6893R)-50ul/100ul/200ul
￥1180 - 2800
