Blank control (blue line): A431 cells(fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃).
_x000D_
Primary Antibody (green line): Rabbit Anti-Phospho-HSP27(Ser78) antibody (bs-3179R);_x000D_
Dilution: 0.2μg /10^6 cells;
_x000D_
Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC;_x000D_
Dilution: 1μg /test.
_x000D_
- ￥1180 - 2800
- Bioss
- bs-3179R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3179R |
| 英文名称 | phospho-HSP27 (Ser78) Rabbit pAb |
| 中文名称 | 磷酸化热休克蛋白27抗体 |
| 英文别名 | Hsp27(phospho S78); p-Hsp27(phospho S78); Heat shock 27kDa protein; 28 kDa heat shock protein; CMT2F; DKFZp586P1322; Estrogen regulated 24 kDa protein; Estrogen-regulated 24 kDa protein; Heat shock 25kDa protein 1; Heat shock 25kDa protein 1; Heat shock 27 kDa protein; Heat shock 27kD protein 1; Heat shock 27kDa protein 1; Heat shock 27kDa protein 1; Heat shock 28kDa protein 1; Heat shock 28kDa protein 1; Heat Shock Protein 27; Heat Shock Protein 27; Heat shock protein beta 1; Heat shock protein beta-1; Heat Shock Protein27; Heat Shock Protein27; HMN2B; HS.76067; Hsp 25; Hsp 25; Hsp 27; Hsp 27; Hsp 28; Hsp 28; Hsp B1; Hsp B1; Hsp25; Hsp25; HSP27; Hsp28; Hsp28; HspB1; HspB1; HSPB1_HUMAN; SRP27; Stress responsive protein 27; Stress-responsive protein 27. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=0.2μg /test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Dog, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human HSP27 around the phosphorylation site of Ser78 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 23 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Tumor biomarkers Cardiovascular > Atherosclerosis > Ischemia / Reperfusion Cardiovascular > Heart > Contractility > Contractile Proteins > Actins Signal Transduction > Protein Trafficking > Chaperones > Heat Shock Proteins |
| 亚基 | Interacts with TGFB1I1. Associates with alpha- and beta-tubulin, microtubules and CRYAB. Interacts with HSPB8 and HSPBAP1. |
| 亚细胞定位 | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles._x000D_ Tissue Specificity : Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle. |
| 组织特异性 | Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle. |
| 翻译后修饰 | Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock. Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress leads to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement. |
| 相似性 | Belongs to the small heat shock protein (HSP20) family. |
| 功能 | Involved in stress resistance and actin organization. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq, Oct 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ICC/IF} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {0.2μg /test} |
Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-HSP27(Ser78)) Polyclonal Antibody, Unconjugated (bs-3179R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
_x000D_ Lane 1: MCF-7 (Human) Cell Lysate at 30 ug
_x000D_ Lane 2: U251 (Human) Cell Lysate at 30 ug
_x000D_ Lane 3: U2os (Human) Cell Lysate at 30 ug
_x000D_ Lane 4: A431 (Human) Cell Lysate at 30 ug
_x000D_ Lane 5: Huvec (Human) Cell Lysate at 30 ug
_x000D_ Lane 6: A549 (Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti-Phospho-HSP27 (Ser78) (bs-3179R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 27 kD
_x000D_ Observed band size: 27 kD
_x000D_ Lane 1: MCF-7 (Human) Cell Lysate at 30 ug
_x000D_ Lane 2: U251 (Human) Cell Lysate at 30 ug
_x000D_ Lane 3: U2os (Human) Cell Lysate at 30 ug
_x000D_ Lane 4: A431 (Human) Cell Lysate at 30 ug
_x000D_ Lane 5: Huvec (Human) Cell Lysate at 30 ug
_x000D_ Lane 6: A549 (Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti-Phospho-HSP27 (Ser78) (bs-3179R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 27 kD
_x000D_ Observed band size: 27 kD
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-HSP27 (Ser78)) polyclonal Antibody, Unconjugated (bs-3179R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Sample:
_x000D_ Muscle (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-Phospho-HSP27(Ser78) (bs-3179R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 23 kD
_x000D_ Observed band size: 23 kD
_x000D_
_x000D_ Muscle (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-Phospho-HSP27(Ser78) (bs-3179R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 23 kD
_x000D_ Observed band size: 23 kD
_x000D_
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HSPB1 | HSP27 (p-S78) Rabbit pAb(bs-3179R)-50ul/100ul/200ul
￥1180 - 2800
