Sample:
Lane 1: Mouse Brown fat tissue lysates
Lane 2: Mouse Heart tissue lysates
Primary: Anti-Adipose Triglyceride Lipase (bs-3831R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 52 kDa
- ￥1180 - 2800
- Bioss
- bs-3831R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3831R |
| 英文名称 | Adipose Triglyceride Lipase Rabbit pAb |
| 中文名称 | 脂肪甘油三酯脂酶抗体 |
| 英文别名 | PEDF R; Adipose Triglyceride Lipase; TTS 2.2; PEDFR; PEDF-R; ATGL; ATGL DESNUTRIN; Calcium independent phospholipase A2; Desnutrin; EC 3.1.1.3; IPLA2 zeta; PATATIN LIKE PHOSPHOLIPASE DOMAIN CONTAINING PROTEIN 2; PHOSPHOLIPASE A2 CALCIUM INDEPENDENT ZETA; Pigment epithelium derived factor; Pnpla2; Transport secretion protein 2; Transport secretion protein 2.2; Triglyceride hydrolase; TTS2; TTS2.2; ZETA. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=3ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human ATGL |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 55 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Lipids / Lipoproteins > Lipid Metabolism > Lipases Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Lipases Metabolism > Types of disease > Metabolic disorders Metabolism > Types of disease > Obesity Signal Transduction > Metabolism > Lipid metabolism |
| 亚基 | Interacts with ABHD5; this association stimulates PNPLA2 triglyceride hydrolase activity. |
| 亚细胞定位 | Lipid droplet. Cell membrane. |
| 组织特异性 | Highest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level). |
| 相似性 | Contains 1 patatin domain. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Adipose triglyceride lipase (ATGL) may function as a lipase and play a role in the adaptive response to a low energy state, such as fasting, by providing fatty acids to other tissues for oxidation. In addition, decreased expression of desnutrin in obesity models suggests its possible contribution to the pathophysiology of obesity. ATGL catalyzes the initial step in triglyceride hydrolysis in mammalian adipose tissue. ATGL contains a patatin domain common to plant acyl hydrolases. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {3ug/Test} |
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-Adipose Triglyceride Lipase antibody (bs-3831R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-Adipose Triglyceride Lipase antibody (bs-3831R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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文献和实验该产品被引用文献
[IF={{ 2.742 }}] {Liu, Yanrong. et al. Cinnamaldehyde affects lipid droplets metabolism after adipogenic differentiation of C2C12 cells. MOL BIOL REP. 2022 Dec;:1-7} {WB} {Mouse}
[IF={{ 2.532 }}] {Chenming Zhang. et al. Transcriptomics and proteomics analysis to explore the mechanism of Yishen Tongluo formula repairing sperm DNA damage in rats. ANDROLOGIA. 2022 Sep;:e14582} {WB} {Rat}
相关实验
Large-Scale Lipoprotein Lipase Purification from Adipose Tissue
Lipoprotein lipase (LPL) plays a central role in lipoprotein metabolism. LPL is the major enzyme responsible for the hydrolysis of triglycerides in VLDL and chylomicrons. Following hydrolysis of the triglyceride core of lipoproteins
Hormone-Sensitive Lipase and Neutral Cholesteryl Ester Lipase
. 1 ). It also catalyzes the hydrolysis of diacylglycerols and monoacylglycerols. However, a second enzyme, monoglyceride lipase, is required to obtain a complete degradation of monoacylglycerols (2 ). HSL is most abundantly expressed in adipose tissue
High-Level Baculoviral Expression of Hormone-Sensitive Lipase
The limited availability of purified hormone-sensitive lipase (HSL) has been one of the major obstacles in working with this enzyme. The low tissue abundance (approx 1 �g HSL/g adipose tissue) and the labile and hydrophobic character of HSL
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Adipose Triglyceride Lipase Rabbit pAb(bs-3831R)-50ul/100ul/200ul
￥1180 - 2800
