Black line : Positive blank control (HL60); Negative blank control (HUVEC）
Green line : Primary Antibody (Rabbit Anti-NCR3 antibody (bs-2418R) )
Orange line：Isotype Control Antibody (Rabbit IgG) .
Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF647)
HL60（Positive）and HUVEC（Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with NCR3 Antibody(bs-2418R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
- ￥1180 - 2800
- Bioss
- bs-2418R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2418R |
| 英文名称 | NCR3 Rabbit pAb |
| 中文名称 | 细胞毒性受体NK-p30（CD337）抗体 |
| 英文别名 | Natural cytotoxicity triggering receptor 3; 1C7; Activating natural killer receptor p30; Activating NK A1 receptor; CD337; CD337 antigen; LY117; Lymphocyte antigen 117; MALS; Natural cytotoxicity triggering receptor 3; Natural cytotoxicity triggering receptor 3; Natural killer cell p30 related protein; Natural killer cell p30 related protein; Natural killer cell p30-related protein; NK-p30; NKp30; NCTR3_HUMAN. |
| 产品应用 | Flow-Cyt=2ug/Test Not yet tested in other applications. |
| 交叉反应 | Human (Rat, Dog, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CD337 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 20 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Immunology > Immunoglobulins > Receptors Stem Cells > Hematopoietic Progenitors > Lymphoid > NK Cell Lineage |
| 亚基 | Homodimer in the unliganted form. Interacts with CD3Z. Interacts with and is activated by binding to B7H6. Interacts with and is activated by binding to BAG6. |
| 亚细胞定位 | Cell membrane; Single-pass type I membrane protein. |
| 组织特异性 | Selectively expressed by all resting and activated NK cells and weakly expressed in spleen. |
| 相似性 | Belongs to the natural cytotoxicity receptor (NCR) family. Contains 1 Ig-like (immunoglobulin-like) domain. |
| 功能 | Cytotoxicity-activating receptor that contributes to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. Engagement of NCR3 by BAG6 also promotes dendritic cell (DC) maturation, both through killing those DCs that did not properly acquire a mature phenotype, and inducing NK cells to release TNFA and IFNG, which promotes DC maturation. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | NCR3 is a cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. It is a member of the Ig superfamily and may cooperate with NKp46 and NKp44 in the induction of cytotoxicity against a variety of target cells and is selectively expressed by all NK cells, both freshly isolated and cultured in IL2, thus representing an optimal marker for NK cell identification. Similar to NKp46 and CD16, NKp30 associated with CD3 zeta chains becomes tyrosine phosphorylated following cell treatment with pervanadate. Its extracelluar portion is characterized by a single domain of the V-type and by a region rich in hydrophobic amino acids, potentially involved in protein/protein interactions that connects the Ig V-like domain with the transmembrane portion. It is selectively expressed by all resting and activated NK cells and weakly expressed in spleen. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {2ug/Test} |
Blank control: HL60.
Primary Antibody (green line): Rabbit Anti-NCR3 antibody (bs-2418R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-NCR3 antibody (bs-2418R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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NCR3 Rabbit pAb(bs-2418R)-50ul/100ul/200ul
￥1180 - 2800
