Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Raw264.7 cell lysates
Lane 3: Mouse NIH/3T3 cell lysates
Lane 4: Rat Testis tissue lysates
Lane 5: Human PANC-1 cell lysates
Primary: Anti-AP2 alpha (bs-3569R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kDa
Observed band size: 52 kDa
- ￥1180 - 2800
- Bioss
- bs-3569R
- 2025年10月24日
- 产品信息以Bioss网站为准
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3569R |
| 英文名称 | AP2 alpha Rabbit pAb |
| 中文名称 | 转录激活蛋白2α/TFAP2α/AP-2α抗体 |
| 英文别名 | Activating enhancer binding protein 2 alpha; Activating enhancer-binding protein 2-alpha; Activator protein 2; AP 2; AP 2 transcription factor; AP 2alpha; AP-2; AP-2 transcription factor; AP2; AP2 Transcription Factor; AP2-alpha; AP2A_HUMAN; AP2TF; BOFS; Clathrin Adaptor Protein Complex; FLJ51761; TFAP 2; TFAP 2A; TFAP2; TFAP2A; Transcription factor AP 2 alpha(activating enhancer binding protein 2 alpha); Transcription factor AP 2 alpha; Transcription factor AP-2-alpha; Transcription factor AP2 alpha. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human AP2 alpha |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 48 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Transcription > Other factors |
| 亚基 | Binds DNA as a dimer. Can form homodimers or heterodimers with other AP-2 family members. Interacts with WWOX. Interacts with CITED4. Interacts with UBE2I. Interacts with RALBP1 in a complex also containing EPN1 and NUMB during interphase and mitosis. Interacts with KCTD1; this interaction represses transcription activation. Interacts (via C-terminus) with CITED2 (via C-terminus); the interaction stimulates TFAP2A-transcriptional activation. Interacts (via N-terminus) with EP300 (via N-terminus); the interaction requires CITED2. |
| 亚细胞定位 | Nucleus. |
| 翻译后修饰 | Sumoylated on Lys-10; which inhibits transcriptional activity (Probable). |
| 相似性 | Belongs to the AP-2 family. |
| 功能 | Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验相关实验
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
.g., Sigma) 50 mg/ml bis‐sulfosuccinimdylsuberate (BS3 ;Pierce, cat. no. PI 21580) in dimethylsulfoxide (DMSO) 5 mg/ml
-2细胞的增殖，诱导调亡。采用的基因芯片包含156个功能分类的3 800项基因。下调表达的基因中包括RNA聚合酶Ⅱ中的9个成员、转录辅抑制蛋白和增强结合蛋白如AP2相关基因，以及具有锌指结构组转录因子。激活与诱导调亡程序有关的细胞色素C过程中的多种成分。激活细胞周期蛋白依赖激酶抑制因子如p21(wafl/cipl)、p27、p57、p19和生长抑制性蛋白相关基因上调表达2倍以上。而且抑制抗凋亡的Bcl-2家族基因的表达，抑制前列腺家族基因、脂加氧酶的表达。DHA改变过氧化物酶体增生物(PPARα和γ)的表达
Generation of Spatio‐Temporally Controlled Targeted Somatic Mutations in the Mouse
Syringe (1‐ml equipped with a 25‐G needle; Terumo, cat. no. BS‐01 H2516) Gas anesthesia station for rodents (TEM)
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AP2 alpha Rabbit pAb(bs-3569R)-50ul/100ul/200ul
￥1180 - 2800
