Tissue/cell: mouse stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-EPX Polyclonal Antibody, Unconjugated(bs-3881R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-3881R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3881R |
| 英文名称 | EPX Rabbit pAb |
| 中文名称 | 嗜酸性粒细胞过氧化物酶抗体 |
| 英文别名 | Eosinophil peroxidase; Eosinophil peroxidase heavy chain; EPER; EPO; EPP; EPX PEN; PERE; PERE_HUMAN. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Eosinophil peroxidase heavy chain |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 79 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Cellular metabolic process Stem Cells > Hematopoietic Progenitors > Myeloid > Neutrophil Lineage |
| 亚基 | Tetramer of two light chains and two heavy chains. |
| 亚细胞定位 | Cytoplasmic granule. Note=Cytoplasmic granules of eosinophils. |
| 相似性 | Belongs to the peroxidase family. XPO subfamily. |
| 功能 | Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | EPX, eosinophil peroxidase, is an antimycobacterial protein that localizes to cytoplasmic granules of eosinophils and recruits bromide to generate a halogenating oxidant. EPX dependent generation of hypobromous acid causes damage to tissue during inflammatory conditions that include asthma, allergies,cancer and parasitic/helminthic infections. EPX is a major enzyme present in eosinophils and upon degranulation, becomes released into the airways of asthmatics. As a result of its cationic nature and its ability to catalyze the formation of highly toxic oxidants, EPX can induce lung injury in a JNK dependent manner. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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文献和实验该产品被引用文献
[IF={{ 7.3 }}] {Tian Xiao. et al. Palmatine treats urticaria by reducing inflammation and increasing autophagy. FRONT IMMUNOL. 2023; 14: 1268467} {IHC} {Rat}
[IF={{ 5.923 }}] {Geunyeong Kim. et al. Der f 38 Is a Novel TLR4-Binding Allergen Related to Allergy Pathogenesis from Dermatophagoides farinae. Int J Mol Sci. 2021 Jan;22(16):8440} {IHC} {Mouse}
[IF={{ 5.714 }}] {Jialiang Luo. et al. D-Mannose ameliorates DNCB-induced atopic dermatitis in mice and TNF-α-induced inflammation in human keratinocytes via mTOR/NF-κB pathway. INT IMMUNOPHARMACOL. 2022 Dec;113:109378} {IHC} {Mouse}
[IF={{ 5.3 }}] {Seo-Hee Kim. et al. The effect of apigenin, an aryl hydrocarbon receptor antagonist, in Phthalate-Exacerbated eosinophilic asthma model. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2023 Jun;27(13):1900-1910} {WB} {Mouse}
[IF={{ 4.8 }}] {Tian Xiao. et al. Metabolomics combined with molecular docking and dynamics simulation to investigate the mechanism of action of Fibraurea recisa Pierre in the treatment of chronic urticaria. FRONT PHARMACOL. 2025 May;16:} {IHC} {Rat}
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EPX Rabbit pAb(bs-3881R)-50ul/100ul/200ul
￥1180 - 2800
