Blank control: A431.
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Primary Antibody (green line): Rabbit Anti-BDH1 antibody (bs-5041R)
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Dilution: 1μg /10^6 cells;
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Isotype Control Antibody (orange line): Rabbit IgG .
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Secondary Antibody: Goat anti-rabbit IgG-AF647
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Dilution: 1μg /test.
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Protocol
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The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min atroom temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-5041R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-5041R |
| 英文名称 | BDH1 Rabbit pAb |
| 中文名称 | 3-羟丁酸脱氢酶抗体 |
| 英文别名 | 3 hydroxybutyrate dehydrogenase(heart, mitochondrial); BDH; BDH-1; D beta hydroxybutyrate dehydrogenase, mitochondrial; 3-hydroxybutyrate dehydrogenase; D-beta-hydroxybutyrate dehydrogenase; mitochondrial; BDH_HUMAN . |
| 产品应用 | Flow-Cyt=3ug/Test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human BDH1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 33 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of lipids and lipoproteins Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Lipid metabolism Metabolism > Pathways and Processes > Mitochondrial Metabolism > Mitochondrial markers Signal Transduction > Metabolism > Lipid metabolism Signal Transduction > Metabolism > Mitochondrial |
| 亚基 | Homotetramer. |
| 亚细胞定位 | Mitochondrion matrix. |
| 相似性 | Belongs to the short-chain dehydrogenases/reductases (SDR) family. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the short-chain dehydrogenase/reductase gene family. The encoded protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. The encoded protein catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {3ug/Test} |
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[IF=4.225] Li H et al . Trimetazidine Ameliorates Myocardial Metabolic Remodeling in Isoproterenol-Induced Rats Through Regulating Ketone Body Metabolism via Activating AMPK and PPAR α Front Pharmacol. 2020 Aug 14;11:1255. Other
[IF=4.225] Li Huihui. et al. Trimetazidine Ameliorates Myocardial Metabolic Remodeling in Isoproterenol-Induced Rats Through Regulating Ketone Body Metabolism via Activating AMPK and PPAR α. Front Pharmacol. 2020 Aug;11:1255 WB Rat
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BDH1 Rabbit pAb(bs-5041R)-50ul/100ul/200ul
￥1180 - 2800
