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50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-2575R |
| 英文名称 | Glycophorin A Rabbit pAb |
| 中文名称 | 血型糖蛋白A(CD235a)抗体 |
| 英文别名 | AI853584; CD235a; GPA; GYPA; Blood group--MN locus; CD235a antigen; Glycophorin A(MNS blood group); Glycophorin A includes MN blood group; Glycophorin A precursor; Glycophorin A, included; GlycophorinA; GPA; GPErik; GpMiIII; GPSAT; GYPA; GYPA, included; HGpMiIII; HgpMiV; HgpMiX; HgpMiXI; HGpSta(C); MN; MN sialoglycoprotein; MNS; PAS 2; PAS-2; PAS2; Sialoglycoprotein alpha; GLPA_MOUSE. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from mouse GPA |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 14 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Blood > Blood Cell Antigens > RBC Antigens Cardiovascular > Blood > Blood Cell Antigens > RBC Antigens > Blood Group Antigens |
| 亚基 | Homodimer. Interacts with Streptococcus gordonii has protein. |
| 亚细胞定位 | Cell membrane; Single-pass type I membrane protein. Note=Appears to be colocalized with SLC4A1. |
| 翻译后修饰 | The N-terminal extracellular domain is heavily glycosylated on serine and threonine residues. |
| 相似性 | Belongs to the glycophorin A family. |
| 功能 | Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |

Western blot analysis of extracts from bone using Glycophorin antibody. (Primary dilution:1:300)

Sample:
_x000D_ Lane 1: K562 (Human) Cell Lysate at 30 ug
_x000D_ Lane 2: HL60 (Human) Cell Lysate at 30 ug
_x000D_ Lane 3: Molt-4 (Human) Cell Lysate at 30 ug
_x000D_ Lane 4: Jurkat (Human) Cell Lysate at 30 ug
_x000D_ Lane 5: 293T (Human) Cell Lysate at 30 ug
_x000D_ Primary:
_x000D_ Anti-Glycophorin A (bs-2575R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 51 kD
_x000D_ Observed band size: 51 kD
_x000D_ Lane 1: K562 (Human) Cell Lysate at 30 ug
_x000D_ Lane 2: HL60 (Human) Cell Lysate at 30 ug
_x000D_ Lane 3: Molt-4 (Human) Cell Lysate at 30 ug
_x000D_ Lane 4: Jurkat (Human) Cell Lysate at 30 ug
_x000D_ Lane 5: 293T (Human) Cell Lysate at 30 ug
_x000D_ Primary:
_x000D_ Anti-Glycophorin A (bs-2575R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 51 kD
_x000D_ Observed band size: 51 kD

Sample:
_x000D_ Lane 1: Blood cell (Mouse) Lysate at 40 ug
_x000D_ Lane 2: Lung (Mouse) Lysate at 40 ug
_x000D_ Lane 3: Kidney (Mouse) Lysate at 40 ug
_x000D_ Lane 4: Placenta (Mouse) Lysate at 40 ug
_x000D_ Lane 5: Spleen (Mouse) Lysate at 40 ug
_x000D_ Primary:
_x000D_ Anti-Glycophorin A (bs-2575R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 51 kD
_x000D_ Observed band size: 51 kD
_x000D_ Lane 1: Blood cell (Mouse) Lysate at 40 ug
_x000D_ Lane 2: Lung (Mouse) Lysate at 40 ug
_x000D_ Lane 3: Kidney (Mouse) Lysate at 40 ug
_x000D_ Lane 4: Placenta (Mouse) Lysate at 40 ug
_x000D_ Lane 5: Spleen (Mouse) Lysate at 40 ug
_x000D_ Primary:
_x000D_ Anti-Glycophorin A (bs-2575R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 51 kD
_x000D_ Observed band size: 51 kD
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