Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CYP17 Polyclonal Antibody, Unconjugated(bs-3853R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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- bs-3853R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3853R |
| 英文名称 | Cytochrome P450 17A1 Rabbit pAb |
| 中文名称 | 细胞色素P450 17抗体 |
| 英文别名 | CPT7; CYP17; CYP17A1; Cytochrome P450 17A1; CYPXVII; Cytochrome P450 family 17; Cytochrome P450 family 17 subfamily A polypeptide 1; Cytochrome p450 XVIIA1; Cytochrome p450, subfamily XVII(steroid 17 alpha hydroxylase) adrenal hyperplasia; P450 C17; P450c17; S17AH; Steroid 17 alpha hydroxylase/17, 20 lyase; Steroid 17 alpha monooxygenase; CP17A_HUMAN. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Dog, Pig, Cow, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CYP17 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 57 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Hormone biosynthesis Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of lipids and lipoproteins Cancer > Growth factors > Hormones Cardiovascular > Lipids / Lipoproteins > Lipid Metabolism > Cytochromes Metabolism > Pathways and Processes > Endocrine metabolism > Hormone biosynthesis Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Lipases Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Lipid metabolism Metabolism > Pathways and Processes > Mitochondrial Metabolism > Cytochromes Metabolism > Types of disease > Cancer Signal Transduction > Growth Factors/Hormones > Hormones Signal Transduction > Metabolism > Lipid metabolism |
| 亚细胞定位 | Membrane. |
| 翻译后修饰 | Phosphorylation is necessary for 17,20-lyase, but not for 17-alpha-hydroxylase activity. |
| 相似性 | Belongs to the cytochrome P450 family. |
| 功能 | Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Cytochrome P450 17A1 (CYP17A1) belongs to the cytochrome P450 family; it plays a role in the conversion of pregnenolone and progesterone into their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. CYP17A1 also catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. CYP17A1 is involved in sexual development during fetal life and at puberty. Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5). AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP17) Polyclonal Antibody, Unconjugated (bs-3853R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP17) Polyclonal Antibody, Unconjugated (bs-3853R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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文献和实验相关实验
Isolation and Purification of Constitutive Forms of Microsomal Cytochrome P450
from microsomes through the use of proteases were not successful, resulting merely in the solubilization of a number of other microsomal proteins. The breakthrough event was the solubrlizatron of rabbit liver microsomal cytochrome P450 using the ionic detergent
Expression of Eukaryotic Cytochromes P450 in E. coli
Although much has been learned from the study of relatively abundant cytochromes P450 (e.g., P450cam from Pseudomonas putida cytosol, P4501A2 and P4502B4 from rabbit liver microsomes, and P45Oscc from bovine adrenal cortex mitochondria
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Cytochrome P450 17A1 Rabbit pAb(bs-3853R)-50ul/100ul/200ul
￥1180 - 2800
