Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADRA1/ADRA1B/alpha 1 Adrenergic Receptor Polyclonal Antibody, Unconjugated (bs-0600R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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- bs-0600R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0600R |
| 英文名称 | ADRA1A Rabbit pAb |
| 中文名称 | alpha 1肾上腺素能受体A抗体 |
| 英文别名 | ADA1A_HUMAN; Adrenergic alpha 1A receptor; Adrenergic alpha 1C receptor; Adrenergic alpha 1D receptor; alpha 1 Adrenergic Receptor; alpha 1A adrenergic receptor; alpha-1A adrenergic receptor; alpha-1A adrenoreceptor; alpha-1C adrenergic receptor; alpha-adrenergic receptor 1c; ADRA1A; ADRA1C; alpha 1A adrenoceptor; alpha-1A adrenergic receptor isoform 1; adrenergic, alpha-1A-, receptor variant 1; adrenergic, alpha-1A-, receptor variant 3; adrenergic, alpha-1A-, receptor variant 5; adrenergic, alpha-1A-, receptor variant 8; G protein coupled receptor; alpha-1A adrenoceptor; ADRA1L1; ALPHA1AAR. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, GuineaPig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Alpha-1A adrenergic receptor |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 51 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Atherosclerosis > Diabetes associated Cardiovascular > Atherosclerosis > Ischemia / Reperfusion Cardiovascular > Blood > Blood Pressure regulation Cardiovascular > Heart > Cardiac arrhythmias Neuroscience > Neurotransmission > Receptors / Channels > GPCR > Adrenergic Receptors Signal Transduction > Growth Factors/Hormones > Adrenalin Signal Transduction > Signaling Pathway > G Protein Signaling > GPCR |
| 亚基 | Homo- and heterooligomer. Heterooligomerizes with ADRA1B homooligomers in cardiac myocytes. |
| 亚细胞定位 | Nucleus membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note=Location at the nuclear membrane facilitates heterooligomerization and regulates ERK-mediated signaling in cardiac myocytes. Colocalizes with GNAQ, PLCB1 as well as LAP2 at the nuclear membrane of cardiac myocytes. |
| 组织特异性 | Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethral and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart. |
| 翻译后修饰 | C-terminal Ser or Thr residues may be phosphorylated. |
| 相似性 | Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA1A sub-subfamily. |
| 功能 | This alpha-adrenergic receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. Nuclear ADRA1A-ADRA1B heterooligomers regulate phenylephrine(PE)-stimulated ERK signaling in cardiac myocytes. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties. [provided by RefSeq, Jul 2008]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADRA1/ADRA1B/alpha 1 Adrenergic Receptor Polyclonal Antibody, Unconjugated (bs-0600R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADRA1/ADRA1B/alpha 1 Adrenergic Receptor Polyclonal Antibody, Unconjugated (bs-0600R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Rat Heart tissue lysates
Primary: Anti-ADRA1A (bs-0600R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 47 kDa
Lane 1: Mouse Heart tissue lysates
Lane 2: Rat Heart tissue lysates
Primary: Anti-ADRA1A (bs-0600R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 47 kDa
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文献和实验该产品被引用文献
[IF={{ 7.84 }}] {Chen, Li-You, et al. "Early-life sleep deprivation persistently depresses melatonin production and bio-energetics of the pineal gland: potential implications for the development of metabolic deficiency." Brain Structure and Function (2014): 1-14.} {WB} {="Rat"}
[IF={{ 4.9 }}] {Wen Fan. et al. Leonurine Inhibits Hepatic Lipid Synthesis to Ameliorate NAFLD via the ADRA1a/AMPK/SCD1 Axis. INT J MOL SCI. 2024 Jan;25(19):10855} {IF,WB} {Mouse}
[IF={{ 4.37 }}] {Wang, Wenjuan, et al. "Effects of Estradiol Valerate and Remifemin on Norepinephrine Signaling in the Brain of Ovariectomized Rats."Neuroendocrinology 101.2 (2015): 120-132.} {IHC} {="Rat"}
[IF={{ 2.59 }}] {Sun, Tao, et al. "Antihypertensive effect of formononetin through regulating the expressions of eNOS, 5-HT< sub> 2A/1B receptors and α< sub> 1-adrenoceptors in spontaneously hypertensive rat arteries." European Journal of Pharmacology (2013).} {Other} {="Rat"}
[IF={{ 2.532 }}] {Jianchao Ren. et al. Ejaculatory Duct Obstruction Affects Seminal Vesicle Contractile Efficacy and Smooth Muscle Ultrastructure in a Rat Model. ANDROLOGIA. 2023;2023:5022466} {WB} {Rat}
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ADRA1A Rabbit pAb(bs-0600R)-50ul/100ul/200ul
￥1180 - 2800
