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50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-16576R |
| 英文名称 | Transmembrane protein 30A Rabbit pAb |
| 中文名称 | 跨膜蛋白30A抗体 |
| 英文别名 | C6orf67; CDC50A; CC50A_HUMAN; Cell cycle control protein 50A; FLJ10856; TMEM30A. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Pig, Cow, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Transmembrane protein 30A |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 41 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Membrane; Multi-pass membrane protein |
| 相似性 | Belongs to the CDC50/LEM3 family. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Enables aminophospholipid flippase activity. Involved in several processes, including phospholipid transport; positive regulation of transport; and xenobiotic transmembrane transport. Located in endoplasmic reticulum and plasma membrane. Part of phospholipid-translocating ATPase complex. [provided by Alliance of Genome Resources, Nov 2021] |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |


Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Transmembrane protein 30A Polyclonal Antibody, Unconjugated(bs-16576R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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文献和实验[IF={{ 5.8 }}] {Yanpei Hou. et al. Tmem30a Protects Against Podocyte Injury Through Suppression of Pyroptosis. ISCIENCE. 2024 五月 15} {WB,IF,IHC} {Human,Mouse}
[IF={{ 3.4 }}] {Yanpei Hou. et al. Integration of metabolomics and transcriptomics reveals the mechanism of TMEM30A downregulation induced FSGS podocyte injury. AM J PHYSIOL-RENAL. 2025 Feb;(Articles in Press):} {WB,IF} {Mouse}
[IF={{ 2.6 }}] {Tuo Shi. et al. The Synergistic Effect of Zuogui Pill and Eldecalcitol on Improving Bone Mass and Osteogenesis in Type 2 Diabetic Osteoporosis. MED LITH. 2023 Aug;59(8):1414} {WB} {Mouse}
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