Blank control:Jurkat.
_x000D_
Primary Antibody (green line): Rabbit Anti-Iba1 antibody (bs-1342R)
_x000D_
Dilution: 1ug/Test;
_x000D_
Secondary Antibody : Goat anti-rabbit IgG-FITC
_x000D_
Dilution: 0.5ug/Test.
_x000D_
Protocol
_x000D_
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
- ￥1180 - 2800
- Bioss
- bs-1342R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1342R |
| 英文名称 | NRF1 Rabbit pAb |
| 中文名称 | 核呼吸因子-1抗体 |
| 英文别名 | erythroid derived 2; like 1; Locus control region factor 1; Locus control region-factor 1; NF-E2-related factor 1; NF2L1_HUMAN; NFE2 related factor 1; NFE2-related factor 1; NFE2L1; NRF-1; NRF 1; Nuclear factor; Nuclear factor erythroid 2-related factor 1; Nuclear factor erythroid derived 2 like 1; TCF-11; TCF11; Transcription factor 11; Transcription factor HBZ17; Transcription factor LCR F1; Transcription factor LCR-F1. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human NRF1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 55 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > ChIP assays > ChIP antibodies Epigenetics and Nuclear Signaling > Transcription > Other factors Epigenetics and Nuclear Signaling > Transcription > Polymerase associated factors > Pol II Transcription Epigenetics and Nuclear Signaling > Transcription > Transcription Factors Metabolism > Pathways and Processes > Metabolic signaling pathways > Nucleotide metabolism > Molecular processes > Mitochondrial transcription Metabolism > Pathways and Processes > Mitochondrial Metabolism > Mitochondrial Biogenesis |
| 亚基 | Homodimer. Binds DNA as a dimer. Interacts with PPRC1. |
| 亚细胞定位 | Nucleus |
| 组织特异性 | Ubiquitously expressed with strongest expression in skeletal muscle. |
| 翻译后修饰 | Phosphorylation enhances DNA binding. |
| 相似性 | Belongs to the NRF1/Ewg family. |
| 功能 | Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ICC/IF} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/Test} |
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NRF1) polyclonal Antibody, Unconjugated (bs-1342R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-NRF-1 Polyclonal Antibody, Unconjugated(bs-1342R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-NRF-1 Polyclonal Antibody, Unconjugated(bs-1342R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
Sample:
_x000D_ Lane1:Heart (Mouse) Lysate at 30 ug
_x000D_ Lane2:Mucle (Mouse) Lysate at 30 ug
_x000D_ Primary: Anti- NRF-1/TCF11 (bs-1342R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 55 kD
_x000D_ Observed band size: 55 kD
_x000D_
_x000D_ Lane1:Heart (Mouse) Lysate at 30 ug
_x000D_ Lane2:Mucle (Mouse) Lysate at 30 ug
_x000D_ Primary: Anti- NRF-1/TCF11 (bs-1342R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 55 kD
_x000D_ Observed band size: 55 kD
_x000D_
Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NRF1) Polyclonal Antibody, Unconjugated (bs-1342R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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文献和实验该产品被引用文献
[IF=6.2] Ming Lou. et al. Butachlor-Induced BAZ2B Activation Promotes Cardiomyocyte Senescence through cGAS-STING pathway. J AGR FOOD CHEM. 2026;74(11):9761–9773 WB Mouse
[IF=6.117] Yue Shen. et al. Lycopene prevents Di-(2-ethylhexyl) phthalate-induced mitophagy and oxidative stress in mice heart via modulating mitochondrial homeostasis. J NUTR BIOCHEM. 2023 May;115:109285 WB Mouse
[IF=3.974] Ning X et al.Ambient PM2. 5 causes lung injuries and coupled energy metabolic disorder. (2018) Ecotoxicol. Environ. Saf. 170 WB Mouse
[IF=3.95] Yan, Wei, et al. "Acute nitrogen dioxide inhalation induces mitochondrial dysfunction in rat brain." Environmental Research 138 (2015): 416-424. WB ="Rat"
[IF=3.76] Guohua Qin. et al. Sulfur dioxide inhalation stimulated mitochondrial biogenesis in rat brains. TOXICOLOGY. 2012 Oct;300:67 WB ="Rat"
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NRF1 | α-PAL Rabbit pAb(bs-1342R)-50ul/100ul/200ul
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