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- Bioss
- bs-10578R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-10578R |
| 英文名称 | WASF3 Rabbit pAb |
| 中文名称 | Verprolin同源结构域包含蛋白3抗体 |
| 英文别名 | KIAA0900; Protein WAVE-3; Protein WAVE3; SCAR3; Verprolin homology domain containing protein 3; Verprolin homology domain-containing protein 3; WASF3; WASF3_HUMAN; WASP family protein member 3; WAVE3; Wiskott Aldrich syndrome protein family member 3; Wiskott-Aldrich syndrome protein family member 3. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Chicken, Dog, Cow, Horse, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human WASF3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 55 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Signal transduction > G protein signaling > Small G proteins Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Actin Binding Proteins Signal Transduction > Signaling Pathway > G Protein Signaling > Small G Proteins > Regulators |
| 亚基 | Binds actin and the Arp2/3 complex. |
| 亚细胞定位 | Cytoplasm, cytoskeleton. |
| 组织特异性 | Expressed in ovary and brain. |
| 翻译后修饰 | Phosphorylation by ABL1 promotes lamellipodia formation and cell migration. |
| 相似性 | Belongs to the SCAR/WAVE family. Contains 1 WH2 domain. |
| 功能 | Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Downstream effector molecules involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RWASF3) Polyclonal Antibody, Unconjugated (bs-10578) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining._x000D_
_x000D_
_x000D_
Sample: ovary (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-WASF3(bs-10578R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 55 kD
_x000D_ Observed band size: 55 kD
_x000D_
_x000D_ Primary: Anti-WASF3(bs-10578R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 55 kD
_x000D_ Observed band size: 55 kD
_x000D_
Sample:
_x000D_ Lane 1: Mouse Cerebellum tissue lysates
_x000D_ Lane 2: Mouse Cerebrum tissue lysates
_x000D_ Lane 3: Mouse Stomach tissue lysates
_x000D_ Lane 4: Mouse Heart tissue lysates
_x000D_ Lane 5: Rat Cerebellum tissue lysates
_x000D_ Lane 6: Rat Cerebrum tissue lysates
_x000D_ Lane 7: Rat Stomach tissue lysates
_x000D_ Primary: Anti-WASF3 (bs-10578R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 55 kDa
_x000D_ Observed band size: 52 kDa
_x000D_ Lane 1: Mouse Cerebellum tissue lysates
_x000D_ Lane 2: Mouse Cerebrum tissue lysates
_x000D_ Lane 3: Mouse Stomach tissue lysates
_x000D_ Lane 4: Mouse Heart tissue lysates
_x000D_ Lane 5: Rat Cerebellum tissue lysates
_x000D_ Lane 6: Rat Cerebrum tissue lysates
_x000D_ Lane 7: Rat Stomach tissue lysates
_x000D_ Primary: Anti-WASF3 (bs-10578R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 55 kDa
_x000D_ Observed band size: 52 kDa
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文献和实验该产品被引用文献
[IF=4.8] Xue-Li Jiang. et al.Thiazole isomers as potential ALK5 inhibitors alleviate P2X7R-mediated inflammation during liver fibrosis..International Immunopharmacology.2025 Mar 20:153:114472. IF Human,Mouse
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WASF3 | WAVE3 Rabbit pAb(bs-10578R)-50ul/100ul/200ul
￥1180 - 2800
