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产品信息以Bioss网站为准
- 规格:
50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-3682R |
| 英文名称 | Nox1 Rabbit pAb |
| 中文名称 | 有丝分裂氧化酶1抗体 |
| 英文别名 | Mitogenic oxidase 1; MOX1; NADH/NADPH mitogenic oxidase subunit P65 MOX; NADPH oxidase 1; NADPH oxidase 1 variant NOH 1L; NADPH oxidase homolog 1; NOH 1; NOH1; RP1-146H21.1; GP91-2; Mitogenic oxidase(pyridine nucleotide dependent superoxide generating). |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Dog, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Nox1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 62 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Other Antibodies > Oxidative Stress Epigenetics and Nuclear Signaling > DNA / RNA > DNA Damage & Repair > DNA Damage Response Immunology > Adaptive Immunity > T Cells > Cytotoxic Cells Metabolism > Pathways and Processes > Metabolic signaling pathways > Nucleotide metabolism Metabolism > Pathways and Processes > Redox metabolism > Oxidative stress Signal Transduction > Metabolism > Plasma Membrane > Channels |
| 亚基 | NOX1, NOXA1, NOXO1, RAC1 and CYBA forms a functional multimeric complex supporting ROS production. Interacts with NOXA1 and NOXO1. |
| 亚细胞定位 | Cell projection, invadopodium membrane; Multi-pass membrane protein. |
| 组织特异性 | NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH-1S is detected only in colon and colon carcinoma cells. |
| 相似性 | Contains 1 FAD-binding FR-type domain. Contains 1 ferric oxidoreductase domain. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The NOX family NADPH protein oxidases are involved in transfer of electrons accross membranes to generate reactive oxygen species. There are 7 family members - NOX1-5, DUOX1 and DUOX2. Expression of NOX1 is highest in colon tissue; it is also found in vascular tissue and can also been induced by a variety of factors including PDGF. It requires interaction with NOXO1 and NOXA1 for superoxide generation. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |

Primary: Anti- Nox1 (bs-3682R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 62 kD
Observed band size: 57 kD

Testis (Mouse) Lysate at 40 ug
Uterus (Mouse) Lysate at 40 ug
DU145(Human) Cell Lysate at 30 ug
Primary: Anti- Nox1 (bs-3682R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 60 kD

Heart (Mouse) Lysate at 40 ug
Primary: Anti-Nox1 (bs-3682R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62 kD
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文献和实验[IF={{ 9.038 }}] {Xie C et al. Mechanism investigation on Bisphenol S-induced oxidative stress and inflammation in murine RAW264.7 cells: The role of NLRP3 inflammasome, TLR4, Nrf2 and MAPK. J Hazard Mater . 2020 Jul 15;394:122549.} {WB,IF} {mouse}
[IF={{ 8.401 }}] {Mr. Aleksandr Vendrov. et al. Renal NOXA1/NOX1 Signaling Regulates Epithelial Sodium Channel and Sodium Retention in Angiotensin II-induced Hypertension. 2021 Oct 29} {IF,IHC} {Mouse}
[IF={{ 6.1 }}] {Yun, Yang, et al. "Sulfate Aerosols Promote Lung Cancer Metastasis by Epigenetically Regulating the Epithelial-to-Mesenchymal Transition (EMT)." Environmental Science & Technology (2017).} {WB} {="Human"}
[IF={{ 5.307 }}] {Xu Zhou. et al. Magnesium isoglycyrrhizinate ameliorates lipopolysaccharide-induced liver injury by upregulating autophagy and inhibiting inflammation via IL-22 expression. BIOORG CHEM. 2022 Nov;128:106034} {IHC} {Mouse}
[IF={{ 5.243 }}] {Peng Ding. et al. BMPER alleviates ischemic brain injury by protecting neurons and inhibiting neuroinflammation via Smad3-Akt-Nrf2 pathway. 2021 Dec 14} {WB} {Mouse}
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
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