Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_
Incubation: Anti-Jak3 Polyclonal Antibody, Unconjugated(bs-2808R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
- ￥1180 - 2800
- Bioss
- bs-2808R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2808R |
| 英文名称 | Jak3 Rabbit pAb |
| 中文名称 | 蛋白酪氨酸激酶JAK-3抗体 |
| 英文别名 | JAK 3; JAK L; JAK3 HUMAN; JAKL; Janus kinase 3(a protein tyrosine kinase, leukocyte); Janus Kinase 3; Janus Kinase3; L JAK; Leukocyte janus kinase; LJAK; Protein tyrosine kinase leukocyte; Tyrosine protein kinase JAK3; EC 2.7.10.2. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Dog, Pig, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Jak3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 125 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Oncoproteins/suppressors > Oncoproteins > Signal transducers Cancer > Signal transduction > Protein phosphorylation > Tyrosine kinases Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > STATs Epigenetics and Nuclear Signaling > Transcription > Transcription Factors Signal Transduction > Protein Phosphorylation > Tyrosine Kinases Signal Transduction > Signaling Pathway > Nuclear Signaling > STATs |
| 亚基 | Interacts with STAM2 and MYO18A. Interacts with SHB. |
| 亚细胞定位 | Endomembrane system; Peripheral membrane protein. Cytoplasm. |
| 组织特异性 | In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins. |
| 翻译后修饰 | Tyrosine phosphorylated in response to IL-2 and IL-4. |
| 相似性 | Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily._x000D_
Contains 1 FERM domain._x000D_ Contains 2 protein kinase domains._x000D_ Contains 1 SH2 domain. |
| 功能 | Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease). [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/test} |
Sample:
_x000D_ SP2/0 Cell (Mouse) Lysate at 40 ug
_x000D_ Jurkat Cell (Human) Lysate at 40 ug
_x000D_ K562 Cell (Human) Lysate at 40 ug
_x000D_ Primary: Anti- Jak3 (bs-2808R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 125 kD
_x000D_ Observed band size: 125 kD
_x000D_ SP2/0 Cell (Mouse) Lysate at 40 ug
_x000D_ Jurkat Cell (Human) Lysate at 40 ug
_x000D_ K562 Cell (Human) Lysate at 40 ug
_x000D_ Primary: Anti- Jak3 (bs-2808R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 125 kD
_x000D_ Observed band size: 125 kD
Blank control:Molt-4.
_x000D_ Primary Antibody (green line): Rabbit Anti-Jak3 antibody (bs-2808R)
_x000D_ Dilution: 0.2μg /10^6 cells;
_x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_ Secondary Antibody : Goat anti-rabbit IgG-PE
_x000D_ Dilution: 0.2μg /test.
_x000D_ Protocol
_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
_x000D_ Primary Antibody (green line): Rabbit Anti-Jak3 antibody (bs-2808R)
_x000D_ Dilution: 0.2μg /10^6 cells;
_x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_ Secondary Antibody : Goat anti-rabbit IgG-PE
_x000D_ Dilution: 0.2μg /test.
_x000D_ Protocol
_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Sample:
_x000D_ Lane 1: Mouse Thymus tissue lysates
_x000D_ Lane 2: Rat Lymph node tissue lysates
_x000D_ Lane 3: Human Jurkat cell lysates
_x000D_ Lane 4: Human Hela cell lysates
_x000D_ Lane 5: Human Molt-4 cell lysates
_x000D_ Primary: Anti-Jak3 (bs-2808R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 125 kDa
_x000D_ Observed band size: 115 kDa
_x000D_ Lane 1: Mouse Thymus tissue lysates
_x000D_ Lane 2: Rat Lymph node tissue lysates
_x000D_ Lane 3: Human Jurkat cell lysates
_x000D_ Lane 4: Human Hela cell lysates
_x000D_ Lane 5: Human Molt-4 cell lysates
_x000D_ Primary: Anti-Jak3 (bs-2808R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 125 kDa
_x000D_ Observed band size: 115 kDa
Blank control（black line）:Molt4.
_x000D_ Primary Antibody (green line): Rabbit Anti-Jak3 antibody (bs-2808R)
_x000D_ Dilution:1ug/Test;
_x000D_ Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
_x000D_ Dilution: 0.5ug/Test.
_x000D_ Isotype control（orange line）: Normal Rabbit IgG
_x000D_ Protocol
_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_ _x000D_
_x000D_ Primary Antibody (green line): Rabbit Anti-Jak3 antibody (bs-2808R)
_x000D_ Dilution:1ug/Test;
_x000D_ Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
_x000D_ Dilution: 0.5ug/Test.
_x000D_ Isotype control（orange line）: Normal Rabbit IgG
_x000D_ Protocol
_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_ _x000D_
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JAK3 Rabbit pAb(bs-2808R)-50ul/100ul/200ul
￥1180 - 2800
