Claudin 5 Rabbit pAb(bs-10296R)-50ul/100ul/200ul
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Claudin 5 Rabbit pAb(bs-10296R

)-50ul/100ul/200ul
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  • ￥1180 - 2800
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  • bs-10296R
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-10296R
    英文名称Claudin 5 Rabbit pAb
    中文名称紧密连接蛋白5抗体
    英文别名AWAL; BEC 1; BEC1; Claudin 5(transmembrane protein deleted in velocardiofacial syndrome); Claudin5; Claudin-5; CLDN 5; CLDN5; CPETR L1; CPETRL 1; CPETRL1; TMDVCF; TMVCF; Transmembrane protein deleted in VCFS; Transmembrane protein deleted in velocardiofacial syndrome; Androgen withdrawal and apoptosis induced protein RVP1 like; CLD5_HUMAN.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse (Rat, Pig, Cow, Horse, Rabbit, Sheep)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human Claudin 5
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量23 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Signal Transduction > Cytoskeleton / ECM > Cell Adhesion > Tight Junctions

    亚基Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ.
    亚细胞定位Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
    组织特异性

    Expressed in right lung and 192 other cell types or tissues

    相似性Belongs to the claudin family.
    功能Plays a major role in tight junction-specific obliteration of the intercellular space.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料 This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2008]

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}
    {Flow-Cyt}{2ug/Test}

     

    Claudin 5 Rabbit pAb(bs-10296R
    Blank control: K562.
    Primary Antibody (green line): Rabbit Anti-Claudin 5 antibody (bs-10296R)
    Dilution:2μg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody : Goat anti-rabbit IgG-FITC
    Dilution: 0.5μg /test.
    Protocol
    The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
    Claudin 5 Rabbit pAb(bs-10296R
    Protein: lung(mouse) lysates at 45ug; Primary: Anti-Claudin 5 (bs-10296R) at 1:300;
    Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000;
    ECL excitated the fluorescence;
    Predicted band size :23 kD
    Observed band size :23kD
    Claudin 5 Rabbit pAb(bs-10296R
    P‌‌araformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Claudin 5) Polyclonal Antibody, Unconjugated (bs-10296R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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    图标文献和实验
    该产品被引用文献

    [IF={{ 6.7 }}] {Musen Lin. et al. Protective Effects of Albiflorin on Acetaminophen-Induced Hepatotoxicity: Regulation of Blood–Biliary Barrier Integrity by ATF3. PHYTOMEDICINE. 2025 Apr;:156722} {WB} {Mouse}

    [IF={{ 5.714 }}] {Yingxue Li. et al. ProBDNF signaling is involved in periodontitis-induced depression-like behavior in mouse hippocampus. INT IMMUNOPHARMACOL. 2023 Mar;116:109767} {WB} {Mouse}

    [IF={{ 5.195 }}] {Chun Niu. et al. Pulsatilla decoction improves DSS-induced colitis via modulation of fecal-bacteria-related short-chain fatty acids and intestinal barrier integrity. J ETHNOPHARMACOL. 2023 Jan;300:115741} {WB} {Mouse}

    [IF={{ 5.195 }}] {Shan Luo. et al. Application of omics technology to investigate the mechanism underlying the role of San Hua Tang in regulating microglia polarization and blood-brain barrier protection following ischemic stroke. J ETHNOPHARMACOL. 2023 May;:116640} {WB} {Rat}

    [IF={{ 5.1 }}] {Min Wei. et al. The role of ROS-pyroptosis in PM2.5 induced air-blood barrier destruction. CHEM-BIOL INTERACT. 2023 Dec;386:110782} {WB,IF} {Human}

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    文献支持
    Claudin 5 Rabbit pAb(bs-10296R)-50ul/100ul/200ul
    ￥1180 - 2800