Blank control (Black line): U87MG (Black).
Primary Antibody (green line): Rabbit Anti-SCF antibody (bs-0545R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-0545R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0545R |
| 英文名称 | SCF Rabbit pAb |
| 中文名称 | 干细胞生长因子抗体 |
| 英文别名 | Stem Cell Factor; C kit ligand; Ckit ligand; DKFZp686F2250; KIT ligand; Kitl; KITLG; KL 1; KL1; Mast cell growth factor; MGF; SF; SHEP7; Steel factor; Stem cell factor precursor; SCF_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Goat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SCF |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 31 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Developmental Biology > Embryogenesis > Embryonic stem cells > Surface molecules Immunology > Innate Immunity > Cytokines > CSFs Stem Cells > Embryonic Stem Cells > Surface Molecules |
| 亚基 | Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation. |
| 亚细胞定位 | Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Cell membrane; Single-pass type I membrane protein. Cytoplasm, cytoskeleton. Soluble KIT ligand: Secreted. |
| 相似性 | Belongs to the SCF family. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble form is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/Test} |
Sample:
Lane 1: Lung (Rat) Lysate at 40 ug
Lane 2: Spleen (Rat) Lysate at 40 ug
Lane 3: MCF-7 (Human) Cell Lysate at 30 ug
Lane 4: Hela (Human) Cell Lysate at 30 ug
Primary: Anti-SCF (bs-0545R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 45/35 kD
Lane 1: Lung (Rat) Lysate at 40 ug
Lane 2: Spleen (Rat) Lysate at 40 ug
Lane 3: MCF-7 (Human) Cell Lysate at 30 ug
Lane 4: Hela (Human) Cell Lysate at 30 ug
Primary: Anti-SCF (bs-0545R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 45/35 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCF) Polyclonal Antibody, Unconjugated (bs-0545R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Blank control (Black line): Mouse spleen(Black).
Primary Antibody (green line): Rabbit Anti-SCF antibody (bs-0545R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 10,000 events was performed.
Primary Antibody (green line): Rabbit Anti-SCF antibody (bs-0545R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 10,000 events was performed.
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文献和实验该产品被引用文献
[IF={{ 5.572 }}] {Ozge Goktepe. et al. The effect of different doses of nonylphenol on the blood-testicular barrier integrity, hormone level, and DNA damage in the testes of rats. FOOD CHEM TOXICOL. 2023 Jul;177:113816} {IHC} {Rat}
[IF={{ 5.195 }}] {Di Zhang. et al. Effects of Banxia Xiexin Decoction on apoptosis of interstitial cells of Cajal via regulation of MiR-451-5p: An in vivo and in vitro study. J ETHNOPHARMACOL. 2023 Oct;314:116606} {WB} {Rat}
[IF={{ 4.929 }}] {Zhou L et al.Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in Rats. (2018) Stem Cells Translational Medicine} {WB} {Rat}
[IF={{ 4.7 }}] {Lei Wu. et al. Naringenin Promotes Gastrointestinal Motility in Mice by Impacting the SCF/c-Kit Pathway and Gut Microbiota. FOODS. 2024 Jan;13(16):2520} {WB} {Mouse}
[IF={{ 4.5 }}] {Junnan Wu. et al. Aqueous extracts of Elsholtzia ciliata and Hovenia dulcis ameliorate loperamide-induced constipation in mice by promoting intestinal peristalsis and barrier function and the abundance of intestinal beneficial bacteria. FRONT MICROBIOL. 2025 May;16:} {IHC} {Mouse}
相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
Studies of the Ubiquitin Proteasome System
Assays of E1 and E2 Activity Basic Protocol 1: Thiolester Formation Between Rabbit E1 and Ubiquitin Basic Protocol 2: Thiolester Formation Between E2 and Ubiquitin Binding of Ubiquitin‐Proteasome Proteins Basic Protocol 3: Binding of E2s to E3s Alternate
进口分装 F0252 血清淀粉样蛋白(SAA) ELISA 48T/96T 2000/3000 进口分装 F0253 可溶性细胞表面分化抗原40 配体 (sCD40L) ELISA 48T/96T 2000/3000 进口分装 F0254 ★ 干细胞因子(SCF) ELISA 48T/96T 1200/2000 进口分装 F0255 干细胞因子受体(SCF R) ELISA 48T/96T 2000/3000 进口分装 F
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SCF Rabbit pAb(bs-0545R)-50ul/100ul/200ul
￥1180 - 2800
