Blank control: A549(blue).
Primary Antibody: Rabbit Anti- AQP9/AF647 Conjugated antibody (bs-2060R /AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
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Protocol
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The cells were fixed with 2% Paraformaldehyde (10 min) . The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (bs-2060R/AF647, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed._x000D_
- ￥1180 - 2800
- Bioss
- bs-2060R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2060R |
| 英文名称 | AQP9 Rabbit pAb |
| 中文名称 | 水通道蛋白-9抗体 |
| 英文别名 | aquaporin Protein-9; Aquaporin 9; AQP 9; AQP9; Aquaporin9; HsT 17287; HsT17287; Small solute channel 1; SSC 1; SSC1; Aquaporin-9; AQP-9; Aquaglyceroporin-9; AQP9_HUMAN. |
| 产品应用 | Flow-Cyt=1μg /test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep, GuineaPig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human AQP9 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 32 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Metabolism > Types of disease > Cancer Signal Transduction > Metabolism > Plasma Membrane > Channels |
| 亚细胞定位 | Membrane; Multi-pass membrane protein. |
| 组织特异性 | Highly expressed in peripheral leukocytes. Also expressed in liver, lung, and spleen. |
| 相似性 | Belongs to the MIP/aquaporin (TC 1.A.8) family. |
| 功能 | Forms a channel with a broad specificity. Mediates passage of a wide variety of non-charged solutes including carbamides, polyols, purines, and pyrimidines in a phloretin- and mercury-sensitive manner, whereas amino acids, cyclic sugars, Na(+), K(+), Cl(-), and deprotonated monocarboxylates are excluded. Also permeable to urea and glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | 牋?Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". A new member of AQP family, Aquaporin-9 (AQP9), has been cloned from human leukocytes by homology cloning (Ishibashi et al. 1997). It is mostly homologous with AQP3 and AQP7 (54-48% identity). AQP9 (295 AA) is primarily expressed in peripheral leukocytes, and lesser amounts in liver, lung and spleen. AQP9 is permeable to water and urea. Unlike AQP3 and AQP7, it did not facilitate glycerol transport. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic. A new member of the AQP family, AQP9, has been cloned from human leukocytes. It is homologous with AQP3 and AQP7 (54-48% identity). AQP9 (295aa) is primarily expressed in peripheral leukocytes, and in lesser amounts in liver, lung and spleen. AQP9 is permeable to water and urea. Unlike AQP3 and AQP7, it does not facilitate glycerol transport. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic.牋牋Other Aliases:牋?aquaporin Protein -9; AQP 9; AQP9; Aquaporin9; HsT 17287; HsT17287; Small solute channel 1; SSC 1; SSC1. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1μg /test} |
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文献和实验该产品被引用文献
[IF=4.9] Ji Woo Im. et al. Stichoposide C and Rhizochalin as Potential Aquaglyceroporin Modulators. MAR DRUGS. 2024 Aug;22(8):335 IHC Mouse
[IF=2.7] Maren E. Buenning. et al. Short-Time Alternating Current Electrical Stimulation and Cell Membrane-Related Components. APPL SCI-BASEL. 2024 Jan;14(2):812 IF Human
[IF=2.6] Farrag Mayada Saad. et al. Aquaporin-9 expression as an independent adverse prognostic marker in diffuse large B-cell lymphoma: an immunohistochemical tissue microarray study. J MOL HISTOL. 2026 Jun;57(3):158 IHC Human
[IF=2.2] El-Nablaway Mohammad. et al. Metformin mitigates metabolic dysfunction-associated steatohepatitis in rats by altering steatosis-related micro-RNAs and Aquaporin-9. J MOL HISTOL. 2026 Feb;57(1):59 IHC Rat
[IF=2] Simin Chen. et al. Zengye decoction regulated the expression of aquaporin in colon tissue of rats with constipation through miR-10a-5p targeting Drd2/AC/cAMP axis. HISTOL HISTOPATHOL. 2025 Jan 21:18876 IF Rat
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AQP9 Rabbit pAb(bs-2060R)-50ul/100ul/200ul
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