Tissue/cell: Human esophageal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_
Incubation: Anti-Cathepsin B Polyclonal Antibody, Unconjugated(bs-1500R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
- ￥1180 - 2800
- Bioss
- bs-1500R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1500R |
| 英文名称 | Cathepsin B Rabbit pAb |
| 中文名称 | 组织蛋白酶B抗体 |
| 英文别名 | Amyloid precursor protein secretase; APP secretase; APPS; Cathepsin B; CB; Cathepsin B1; CathepsinB; CPSB; CTSB; Cysteine protease; Preprocathepsin B; Cathepsin B heavy chain; Cathepsin B light chain; CATB_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Cathepsin B heavy chain |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 23/28/37 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Invasion/microenvironment > Angiogenesis > ECM enzymes > Cathespins Cancer > Invasion/microenvironment > ECM > Cell adhesion Cancer > Invasion/microenvironment > ECM > Extracellular matrix Kits/ Lysates/ Other > Kits > ELISA Kits > ELISA Kits > Adhesion molecules ELISA kits Neuroscience > Cell Adhesion Proteins > Membrane Proteins Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > ECM Enzymes > Other Enzymes |
| 亚基 | Dimer of a heavy chain and a light chain cross-linked by a disulfide bond. Interacts with SRPX2. |
| 亚细胞定位 | Lysosome. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| 相似性 | Belongs to the peptidase C1 family. |
| 功能 | Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer disease, the most common cause of dementia. Overexpression of the encoded protein, which is a member of the peptidase C1 family, has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1µg/Test} |
Sample:
_x000D_ Spleen (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-Cathepsin B (bs-1500R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 46/28 kD
_x000D_ Observed band size: 30 kD
_x000D_ Spleen (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-Cathepsin B (bs-1500R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 46/28 kD
_x000D_ Observed band size: 30 kD
Blank control: RSC96(blue).
Primary Antibody:Rabbit Anti- Cathepsin B antibody(bs-1500R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
_x000D_ _x000D_ Protocol
_x000D_ The cells were fixed with 2% Paraformaldehyde (10 min) . Antibody (bs-1500R, 5μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-1500R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed._x000D_
Primary Antibody:Rabbit Anti- Cathepsin B antibody(bs-1500R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
_x000D_ _x000D_ Protocol
_x000D_ The cells were fixed with 2% Paraformaldehyde (10 min) . Antibody (bs-1500R, 5μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-1500R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed._x000D_
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CTSB | Cathepsin B Rabbit pAb(bs-1500R)-50ul/100ul/200ul
￥1180 - 2800
