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- 2025年10月24日
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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1544R |
| 英文名称 | Shh Rabbit pAb |
| 中文名称 | Shh信号转导通路膜蛋白受体抗体 |
| 英文别名 | Sonic hedgehog; Vhh-1; Sonic hedgehog protein; SHH; HHG 1; HHG1; HLP 3; HLP3; Holoprosencephaly 3; HPE 3; HPE3; MCOPCB5; SMMC I; SMMCI; Sonic Hedgehog(Drosophila) homolog; sonic hedgehog homolog(Drosophila); Sonic hedgehog homolog; Sonic hedgehog protein; TPT; TPTPS; Sonic hedgehog protein N-product; SHH_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Chicken, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Shh |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 19/47 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Interacts with HHATL/GUP1 which negatively regulates HHAT-mediated palmitoylation of the SHH N-terminus. N-product is active as a multimer. |
| 亚细胞定位 | Sonic hedgehog protein C-product: Secreted, extracellular space. Note=The C-terminal peptide diffuses from the cell. Sonic hedgehog protein N-product: Cell membrane; Lipid-anchor. Note=The N-product either remains associated with lipid rafts at the cell surface, or forms freely diffusible active multimers with its hydrophobic lipid-modified N- and C-termini buried inside. |
| 组织特异性 | Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues. |
| 翻译后修饰 | The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity. Cholesterylation is required for N-product targeting to lipid rafts and multimerization (By similarity). N-palmitoylation of Cys-24 by HHAT is required for N-product multimerization and full activity (By similarity). |
| 相似性 | Belongs to the hedgehog family. |
| 功能 | Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction (By similarity). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | SHH binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction (By similarity). |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Primary: Anti-Shh (bs-1544R) at 1:300 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size : 19/47kD
Observed band size : 50kD
Primary: Anti-Shh(bs-1544R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 19/47kD
Observed band size: 49 kD
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文献和实验[IF={{ 8.98 }}] {Weiss, Robert M., et al. "Brg1 Determines Urothelial Cell Fate during Ureter Development." Journal of the American Society of Nephrology: JASN (2013).} {Other} {=""}
[IF={{ 8.718 }}] {Chao Sun. et al. WISP-1 induced by mechanical stress contributes to fibrosis and hypertrophy of the ligamentum flavum through Hedgehog-Gli1 signaling. Exp Mol Med. 2021 Jun;53(6):1068-1079} {WB} {Human}
[IF={{ 5.682 }}] {Qi, Yan. et al. Neuroprotective Effect of Sonic Hedgehog Mediated PI3K/AKT Pathway in Amyotrophic Lateral Sclerosis Model Mice. MOL NEUROBIOL. 2022 Sep;:1-12} {WB} {Mouse}
[IF={{ 4.7 }}] {Lili Lu. et al.Hypoxia-inducible factor-1 alpha (HIF-1α) inhibitor AMSP-30 m attenuates CCl₄-induced liver fibrosis in mice by inhibiting the sonic hedgehog pathway..CHEMICO-BIOLOGICAL INTERACTIONS.2025 Mar 18:413:111480.} {Western blot} {Mouse}
[IF={{ 4.56 }}] {Wang, H., et al. "Baicalin Attenuates Alcoholic Liver Injury through Modulation of Hepatic Oxidative Stress, Inflammation and Sonic Hedgehog Pathway in Rats." Cellular Physiology and Biochemistry 39.3 (2016): 1129-1140.} {WB} {="Rat"}
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
are needed to reveal the important role of the members of the Wnt signaling pathway in the RA pathogenesis and treatment. Rspo 1 promotes osteoblast differentiation via Wnt signaling pathway. 作者：Sharma AR, Choi BS, Park JM, Lee DH, Lee JE, Kim HS
/96T 2000/3000 进口分装 F0021 骨成型蛋白-9(BMP-9) ELISA 48T/96T 2000/3000 进口分装 F0022 BMP-R1A ELISA 48T/96T 2000/3000 进口分装 F0023 BMP-R2 ELISA 48T/96T 2000/3000 进口分装 F0024 BTC ELISA 48T/96T 2000/3100 进口分装 F0030 CAE-Ab ELISA 96
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