Sample:
Lane 1: Lymph node (Mouse) Lysate at 30 ug
Lane 2: Thymus (Rat) Lysate at 40 ug
Lane 3: Spleen (Rat) Lysate at 40 ug
Lane 4: Lymph node (Rat) Lysate at 30 ug
Primary:
Anti-CD27 (bs-2491R) at 1/1000 dilution
Anti-beta-Actin (bs-0061R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50-55 kD
Observed band size: 60 kD
- ￥1180 - 2800
- Bioss
- bs-2491R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2491R |
| 英文名称 | CD27 Rabbit pAb |
| 中文名称 | CD27抗体 |
| 英文别名 | CD 27; CD27 antigen; CD27 molecule; CD27_HUMAN; CD27L receptor; LPFS2; MGC20393; OTTHUMP00000238557; S152; T cell activation antigen CD27; T cell antivation antigen S152; T-cell activation antigen CD27; T14; TNFRSF 7; TNFRSF7; TNFSF7; Tp 55; Tp55; Tumor necrosis factor receptor superfamily member 7. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Dog, Pig, Cow) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CD27 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 27 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Tumor immunology > CD markers Cancer > Tumor immunology > Cytokines > TNF Immunology > Adaptive Immunity > Regulatory T Cells Immunology > Adaptive Immunity > T Cells > CD Immunology > Innate Immunity > Cytokines > TNF Superfamily Stem Cells > Hematopoietic Progenitors > Lymphoid > T Lymphocytic Lineage |
| 亚基 | Homodimer. Interacts with SIVA1 and TRAF2. |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 组织特异性 | Found in most T-lymphocytes. |
| 翻译后修饰 | Phosphorylated. O-glycosylated with core 1 or possibly core 8 glycans. |
| 相似性 | Contains 3 TNFR-Cys repeats. |
| 功能 | Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验该产品被引用文献
[IF={{ 1.396 }}] {Xiong Y et al. Functions of T-cell subsets and their related cytokines in the pathological processes of autoimmune encephalomyelitic mice.(2018) Int J Clin Exp Pathol;11(10):4817-4826.} {FCM,IHC} {Mouse}
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CD27 Rabbit pAb(bs-2491R)-50ul/100ul/200ul
￥1180 - 2800
