Tissue/cell: rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IL-17/IL-17A Polyclonal Antibody, Unconjugated(bs-1183R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-1183R
- 2025年10月16日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1183R |
| 英文名称 | IL17A Rabbit pAb |
| 中文名称 | 白介素-17抗体 |
| 英文别名 | Interleukin-17A; CTLA-8; CTLA 8; CTLA8; Cytotoxic T lymphocyte associated antigen 8; Cytotoxic T lymphocyte associated serine esterase 8; IL 17A; IL-17A; IL-17; Interleukin 17; Interleukin-17; Interleukin 17A; Interleukin17; Interleukin17A; IL17_HUMAN; IL17_MOUSE. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from mouse IL-17 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 15 kDa |
| 实际分子量 | 15-17 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 |
Cancer > Tumor immunology > Cytokines > Interleukins Immunology > Innate Immunity > Cytokines > Interleukins |
| 亚基 | Homodimer. |
| 亚细胞定位 | Secreted. |
| 组织特异性 | Restricted to a subset of activated T-cells. |
| 翻译后修饰 | Found both in glycosylated and nonglycosylated forms. |
| 相似性 | Belongs to the IL-17 family. |
| 功能 | Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene is a member of the IL-17 receptor family which includes five members (IL-17RA-E) and the encoded protein is a proinflammatory cytokine produced by activated T cells. IL-17A-mediated downstream pathways induce the production of inflammatory molecules, chemokines, antimicrobial peptides, and remodeling proteins. The encoded protein elicits crucial impacts on host defense, cell trafficking, immune modulation, and tissue repair, with a key role in the induction of innate immune defenses. This cytokine stimulates non-hematopoietic cells and promotes chemokine production thereby attracting myeloid cells to inflammatory sites. This cytokine also regulates the activities of NF-kappaB and mitogen-activated protein kinases and can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). IL-17A plays a pivotal role in various infectious diseases, inflammatory and autoimmune disorders, and cancer. High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL17A. [provided by RefSeq, Sep 2020] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Tissue/cell: rat tongue tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IL-17/IL-17A Polyclonal Antibody, Unconjugated(bs-1183R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IL-17/IL-17A Polyclonal Antibody, Unconjugated(bs-1183R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Sample:
Lane 1: Rat Lymph node tissue lysates
Lane 2: Mouse Lymph node tissue lysates
Lane 3: Rat Thymus tissue lysates
Primary: Anti-IL17A (bs-1183R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kD
Observed band size: 22 kD
Lane 1: Rat Lymph node tissue lysates
Lane 2: Mouse Lymph node tissue lysates
Lane 3: Rat Thymus tissue lysates
Primary: Anti-IL17A (bs-1183R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 15 kD
Observed band size: 22 kD
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文献和实验该产品被引用文献
[IF={{ 8.786 }}] {Xiaoyu Guo. et al. Effects of Boric Acid Gel on Vaginal Candida albicans Infections and the Local Immune System in Mice. FRONT IMMUNOL. 2022; 13: 950215} {IHC} {Mouse}
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[IF={{ 7 }}] {L.H. Frommherz. et al. Secukinumab for the treatment of SAM syndrome associated with desmoglein‐1 deficiency. 2020 Nov 18} {IHC} {Human}
[IF={{ 6.8 }}] {Bing Li. et al. Vitamin D3 reverses immune tolerance and enhances the cytotoxicity of effector T cells in coal pneumoconiosis. ECOTOX ENVIRON SAFE. 2024 Feb;271:115972} {IHC} {Mouse}
[IF={{ 6.7 }}] {Jing-yi Hou. et al. Chuanxiong Qingnao Granules (CQG) alleviates nitroglycerin-induced migraine-like pain in rats by glycerophospholipid metabolism and PI3K/Akt signaling pathway. PHYTOMEDICINE. 2025 Jan;136:156336} {IF} {Rat}
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IL17A Rabbit pAb(bs-1183R)-50ul/100ul/200ul
￥1180 - 2800
