Blank control: Mouse brain cells (blue).
Primary Antibody:Rabbit Anti- Substance P antibody(bs-0065R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% Paraformaldehyde (10 min). Primary antibody (bs-0065R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-0065R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0065R |
| 英文名称 | Substance P Rabbit pAb |
| 中文名称 | P物质抗体 |
| 英文别名 | Hs.2563; Neurokinin 1; Neurokinin 2; Neurokinin A; Neurokinin alpha; Neuromedin L; Neuropeptide gamma; NKA; NKNA; PPT; Protachykinin 1 precursor; Substance K; Substance P; TAC1; TAC2; Tachykinin 1; Tachykinin 2; Tachykinin precursor 1; Tachykinin1; TACI; TKN1_HUMAN; |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Pig, Cow, Horse, Rabbit, Sheep, GuineaPig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Substance P (RPKPQQFFGLM) |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 1.4/13 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Metabolism > Types of disease > Cancer Neuroscience > Neurology process > Metabolism Neuroscience > Neurotransmitter > Neuropeptides > Hormones Neuroscience > Neurotransmitter > Neuropeptides > Tachykinins Neuroscience > Sensory System > Somatosensory system > Nociception |
| 亚细胞定位 | Secreted. |
| 相似性 | Belongs to the tachykinin family. |
| 功能 | Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes four products of the tachykinin peptide hormone family, substance P and neurokinin A, as well as the related peptides, neuropeptide K and neuropeptide gamma. These hormones are thought to function as neurotransmitters which interact with nerve receptors and smooth muscle cells. They are known to induce behavioral responses and function as vasodilators and secretagogues. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] This anitbody is crose activity with Neurokinin B. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ICC/IF} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1μg/Test} |
Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ( Substance P) Polyclonal Antibody, Unconjugated (bs-0065R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
U-2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Substance P) polyclonal Antibody, Unconjugated (bs-0065R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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文献和实验该产品被引用文献
[IF={{ 5.81 }}] {Shengyan Xi. et al. The Ciji-Hua’ai-Baosheng II Formula Attenuates Chemotherapy-Induced Anorexia in Mice With H22 Hepatocellular Carcinoma. Front Pharmacol. 2021; 12: 715824} {IHC} {Mouse}
[IF={{ 5.4 }}] {Qian Yu. et al. The beneficial effect of Sanhuang ointment and its active constituents on experimental hemorrhoids in rats. J ETHNOPHARMACOL. 2024 Jan;319:117173} {IHC} {Rat}
[IF={{ 5.195 }}] {Qian Yu. et al. Yuhong ointment ameliorates inflammatory responses and wound healing in scalded mice. J ETHNOPHARMACOL. 2023 Apr;306:116118} {IHC} {Mouse}
[IF={{ 4.85 }}] {Daschil, Nina, et al. "L-Type calcium channel blockers and substance P induce angiogenesis of cortical vessels associated with beta-amyloid plaques in an Alzheimer mouse model." Neurobiology of Aging (2015).} {IHC} {="Mouse"}
[IF={{ 4.3 }}] {Masakazu Agetsuma. et al. Minimally invasive, wide-field two-photon imaging of the brainstem at cellular resolution. Cell Reports Methods. 2025 Apr;5:} {IHC} {Mouse}
相关实验
ELISA Procedure for Measuring Serum Antibody Titer
to bind. The second antibody usually has an enzyme conjugated to it. This enzyme catalyzes the formation of colored substance, e.g., p-nitrophenol, from a colorless substrate, p-nitrophenylphosphate (Figure 21). This colored substance is then quantified
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
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Substance P Rabbit pAb(bs-0065R)-50ul/100ul/200ul
￥1180 - 2800
