Sample:
Kidney(Mouse) lysate at 30ug;
Brain(Rat) lysate at 30ug;
Primary: Anti-ER-beta (bs-0116R) at 1:200;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000;
Predicted band size : 58kD
Observed band size : 58kD
- ￥1180 - 2800
- Bioss
- 北京
- bs-0116R
- 2025年10月15日
- WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:400-800,IHC-F=1:400-800,Flow Cyt=3μg/Test,IF=1:100-500,
- Rabbit
- Human,Mouse,Rat,Pig,Cow,
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
KLH conjugated synthetic peptide derived from human ER-beta
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 保存条件：
Store at -20 °C for one year.
- 克隆性：
单克隆
- 标记物：
无
- 适应物种：
Human,Mouse,Rat,Pig,Cow,
- 保质期：
1年
- 目录编号：
bs-0116R
- 库存：
999
- 供应商：
北京博奥森生物技术有限公司
- 宿主：
Rabbit
- 应用范围：
WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:400-800,IHC-F=1:400-800,Flow Cyt=3μg/Test,IF=1:100-500,
- 浓度：
1mg/ml
- 抗体英文名：
Estrogen receptor beta
- 抗体名：
雌激素受体β 抗体
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.00 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.00 |
| 规格： | 200ul | 产品价格： | ￥2800.00 |
| 产品编号 | bs-0116R |
| 英文名称 | Estrogen receptor beta Rabbit pAb |
| 中文名称 | 雌激素受体β 抗体 |
| 英文别名 | Estrogen receptor-beta; Estrogen receptor beta; ER BETA; ER-BETA; ER-beta; Erb; ESR 2; ESR 2; ESR B; ESR-B; ESR BETA; ESR-BETA; ESR2; ESR2; ESR2_HUMAN; ESRB; ESTRB; estrogen nuclear receptor beta variant a; estrogen nuclear receptor beta variant b; estrogen receptor 2 (ER beta); Estrogen Receptor 2; Estrogen Receptor-2; estrogen receptor beta 4; NR3A2; Nuclear receptor subfamily 3 group A member 2. |
| 产品应用 | WB=1:500-2000, IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human ER-beta |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 58 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Signal transduction > Nuclear signaling > Nuclear hormone receptors > Estrogen Cancer > Tumor biomarkers > Receptors Epigenetics and Nuclear Signaling > ChIP assays > ChIP kits Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > Nuclear Receptors > Estrogen Epigenetics and Nuclear Signaling > Transcription > Domain Families > Zinc Finger Neuroscience > Endocrine system > Gonadotrophic axis Signal Transduction > Signaling Pathway > Nuclear Signaling > Nuclear Hormone Receptors > Estrogen |
| 亚基 | Interacts with SLC30A9 (By similarity). Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NCOA7 in a ligand-inducible manner. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with KDM5A. Interacts with SMARD1. Interacts with HEXIM1 and MAP1S. Interacts with PBXIP1. Interaction with MUC1 is stimulated by 7 beta-estradiol (E2) and enhances ERS1-mediated transcription. Interacts with DNTTIP2, FAM120B and UIMC1. Interacts with isoform 4 of TXNRD1. Interacts with MLL2. Interacts with ATAD2 and this interaction is enhanced by estradiol. |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Tissue specificityIsoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain. |
| 翻译后修饰 | Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked. |
| 相似性 | Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. |
| 功能 | Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a member of the family of estrogenreceptors and superfamily of nuclear receptor transcriptionfactors. The gene product contains an N-terminal DNA binding domainand C-terminal ligand binding domain and is localized to thenucleus, cytoplasm, and mitochondria. Upon binding to17beta-estradiol or related ligands, the encoded protein formshomo- or hetero-dimers that interact with specific DNA sequences toactivate transcription. Some isoforms dominantly inhibit theactivity of other estrogen receptor family members. Severalalternatively spliced transcript variants of this gene have beendescribed, but the full-length nature of some of these variants hasnot been fully characterized. [provided by RefSeq, Jul 2008]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:400-800} |
| {IHC-F} | {1:400-800} |
| {IF} | {1:100-500} |
| {ELISA} | {1:5000-10000} |
Positive control: Hela
Isotype Control Antibody: Rabbit IgG ; Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 3μg in 100 μL1X PBS containing 0.5% BSA.
Isotype Control Antibody: Rabbit IgG ; Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 3μg in 100 μL1X PBS containing 0.5% BSA.
Sample:
Lane 1: Testis (Mouse) Lysate at 40 ug
Lane 2: Testis (Rat) Lysate at 40 ug
Lane 3: Ovary (Mouse) Lysate at 40 ug
Lane 4: Uterus (Mouse) Lysate at 40 ug
Lane 5: Uterus (Rat) Lysate at 40 ug
Primary: Anti-Estrogen receptor beta (bs-0116R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58-62 kD
Lane 1: Testis (Mouse) Lysate at 40 ug
Lane 2: Testis (Rat) Lysate at 40 ug
Lane 3: Ovary (Mouse) Lysate at 40 ug
Lane 4: Uterus (Mouse) Lysate at 40 ug
Lane 5: Uterus (Rat) Lysate at 40 ug
Primary: Anti-Estrogen receptor beta (bs-0116R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58-62 kD
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-Estrogen receptor beta antibody (bs-0116R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-Estrogen receptor beta antibody (bs-0116R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (bs-0116R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (bs-0116R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (bs-0116R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (bs-0116R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (bs-0116R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (bs-0116R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (bs-0116R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (bs-0116R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (bs-0116R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ESR2 Polyclonal Antibody, Unconjugated (bs-0116R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Cerebellum tissue lysates
Lane 4: Rat Testis tissue lysates
Lane 5: Rat Cerebrum tissue lysates
Lane 6: Rat Cerebellum tissue lysates
Lane 7: Human MCF-7 cell lysates
Lane 8: Human Jurkat cell lysates
Lane 9: Human U-2 OS cell lysates
Lane 10: Human A431 cell lysates
Primary: Anti-ESR2 (bs-0116R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 63 kDa
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Cerebellum tissue lysates
Lane 4: Rat Testis tissue lysates
Lane 5: Rat Cerebrum tissue lysates
Lane 6: Rat Cerebellum tissue lysates
Lane 7: Human MCF-7 cell lysates
Lane 8: Human Jurkat cell lysates
Lane 9: Human U-2 OS cell lysates
Lane 10: Human A431 cell lysates
Primary: Anti-ESR2 (bs-0116R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 63 kDa
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文献和实验该产品被引用文献
[IF={{ 1.91 }}] {Fang, Ming, Xin-Chi Wu, and Wenlong Huang. "Raloxifene Upregulated Mesangial Cell MMP-2 Activity via ER-?? Through Transcriptional Regulation."Cell Biochemistry and Biophysics (2013): 1-7..} {WB} {="Mouse"}
[IF={{ 4.2 }}] {Wei, Qing, et al. "Estrogen suppresses hepatocellular carcinoma cells through ERβ-mediated upregulation of the NLRP3 inflammasome." Laboratory Investigation 95.7 (2015): 804-816.} {IHC} {="Human"}
[IF={{ 4.561 }}] {Rárová,et al.The novel brassinosteroid analog BR4848 inhibits angiogenesis in human endothelial cells and induces apoptosis in human cancer cells in vitro.(2018) The Journal of Steroid Biochemistry and Molecular Biology. 178:263-271.} {WB,ICC} {Human}
[IF={{ 2.561 }}] {Liu R et al. ERβ modulates genistein's cisplatin-enhancing activities in breast cancer MDA-MB-231 cells via P53-independent pathway. Mol Cell Biochem. 2019 Feb 8.} {WB} {Human}
[IF={{ 2.299 }}] {Gao XX et al. Effects of l-arginine on endometrial estrogen receptor α/β and progesterone receptor expression in nutrient-restricted sheep. Theriogenology. 2019 Jul 25;138:137-144.} {IHC,IF,WB} {ewe}
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anti-Estrogen receptor beta
￥1180 - 2800
