Blank control:K562.
Primary Antibody (green line): Rabbit Anti-S100A7 antibody (bs-6238R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-6238R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-6238R |
| 英文名称 | S100A7 Rabbit pAb |
| 中文名称 | S100钙结合蛋白A7抗体 |
| 英文别名 | HID 5; Protein S100 A7; Protein S100-A7; PSOR 1; PSOR1; Psoriasin 1; Psoriasin; Psoriasin1; S100 Calcium binding protein A7; S100 calcium-binding protein A7; S100A7; S100A7c; S10A7_HUMAN; S100A7A; S100A15. |
| 产品应用 | Flow-Cyt=2ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Hela (Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human S100A7 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 11 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Signal transduction Cancer > Tumor biomarkers Signal Transduction > Signaling Pathway > Calcium Signaling > Calcium Binding Proteins Tags & Cell Markers > Cell Type Markers > Tumor Associated |
| 亚基 | Interacts with RANBP9. |
| 亚细胞定位 | Cytoplasm. Secreted. Note=Secreted by a non-classical secretory pathway. |
| 组织特异性 | Fetal ear, skin, and tongue and human cell lines. Highly up-regulated in psoriatic epidermis. Also highly expressed in the urine of bladder squamous cell carcinoma (SCC) bearing patients. |
| 相似性 | Belongs to the S-101 family. Contains 2 EF-hand domains. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Fetal ear, skin, and tongue and human cell lines. Highly up-regulated in psoriatic epidermis. Also highly expressed in the urine of bladder squamous cell carcinoma (SCC) bearing patients. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {2ug/Test} |
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文献和实验该产品被引用文献
[IF={{ 2.4 }}] {Yutong Yan. et al. The Relationship between Mastitis and Antimicrobial Peptide S100A7 Expression in Dairy Goats. Veterinary Sciences. 2023 Nov;10(11):653} {IHC} {Sheep}
[IF={{ 2.141 }}] {Yutong Yan. et al. Immunolocalization of antibacterial peptide S100A7 in mastitis goat mammary gland and lipopolysaccharide induces the expression and secretion of S100A7 in goat mammary gland epithelial cells via TLR4/NFκB signal pathway. ANIM BIOTECHNOL. 2022 Aug 22} {IHC} {Goat}
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S100A7 Rabbit pAb(bs-6238R)-50ul/100ul/200ul
￥1180 - 2800
