Blank control (blue line): Hep G2(blue).
Primary Antibody (green line): Rabbit Anti-CD133 antibody (bs-0395R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol Overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-0395R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0395R |
| 英文名称 | CD133 Rabbit pAb |
| 中文名称 | 造血干细胞抗原CD133抗体 |
| 英文别名 | AC133; Antigen AC133; Hematopoietic stem cell antigen; hProminin; PROM1; Prominin I; Prominin like protein 1 precursor; Prominin mouse like 1; prominin1; PROML1; CORD12; MCDR2; MSTP061; PROML1; RP41; STGD4; PROM1_HUMAN; CD133 antigen. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Fruit Fly) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CD133 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 95 kDa |
| 实际分子量 | 120kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Cell Type Marker > Neural Stem Cell marker Stem Cells > Hematopoietic Progenitors > Surface Molecules Stem Cells > Neural Stem Cells > Surface Molecules |
| 亚基 | Interacts with CDHR1 and with actin filaments. |
| 亚细胞定位 | Cell projection, cilium, photoreceptor outer segment. Isoform 1: Apical cell membrane; Multi-pass membrane protein. Cell projection, microvillus membrane; Multi-pass membrane protein. Note=Found in extracellular membrane particles in various body fluids such as cerebrospinal fluid, saliva, seminal fluid and urine. |
| 组织特异性 | Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level). |
| 翻译后修饰 | Isoform 1 and isoform 2 are glycosylated. |
| 相似性 | Belongs to the prominin family. |
| 功能 | Binds cholesterol in cholesterol-containing plasma membrane microdomains. Proposed to play a role in apical plasma membrane organization of epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {1μg/Test} |
Sample:
SP2/0 (mouse)cell Lysate at 40 ug
Uterus (mouse) Lysate at 40 ug
Lovo (human)cell Lysate at 40 ug
Primary: Anti- CD133 (bs-0395R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95kD
Observed band size: 115 kD
SP2/0 (mouse)cell Lysate at 40 ug
Uterus (mouse) Lysate at 40 ug
Lovo (human)cell Lysate at 40 ug
Primary: Anti- CD133 (bs-0395R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95kD
Observed band size: 115 kD
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文献和实验该产品被引用文献
[IF={{ 8.38 }}] {Alamgeer, Muhammad, et al. "The prognostic significance of aldehyde dehydrogenase 1A1 (ALDH1A1) and CD133 expression in early stage non-small cell lung cancer."?Thorax?(2013).} {IHC} {Human}
[IF={{ 8.2 }}] {Zhiming Shen. et al. Construction of a novel cell-free tracheal scaffold promoting vascularization for repairing tracheal defects. MATER TODAY BIO. 2023 Dec;23:100841} {IF} {Rabbit}
[IF={{ 2.81 }}] {Wang et al. The synergistic in vitro and in vivo antitumor effect of combination therapy with salinomycin and 5-fluorouracil against hepatocellular carcinoma. (2014) PLoS.On. 9:e97414} {IHC} {Mouse}
[IF={{ 2.66 }}] {Chen et al. Co-expression of CD133, CD44v6 and human tissue factor is associated with metastasis and poor prognosis in pancreatic carcinoma. (2014) Oncol.Re. 32:755-63} {IHC} {Human}
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CD133 Rabbit pAb(bs-0395R)-50ul/100ul/200ul
￥1180 - 2800
