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Caspase-3 Rabbit pAb(bs-0081R)

-50ul/100ul

价格￥1180 - 1980

品牌Bioss
货号bs-0081R
更新日期2025年10月01日

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应用范围：
产品信息以Bioss网站为准
规格：
50ul/100ul

规格：	50ul	产品价格：	￥1180.0
规格：	100ul	产品价格：	￥1980.0

产品编号	bs-0081R
英文名称	Caspase-3 Rabbit pAb
中文名称	半胱胺酸蛋白酶蛋白-3抗体
英文别名	Caspase-3 subunit p17; cleaved Caspase 3; cleaved Caspase-3; APOPAIN; CASP3; Caspase 3 apoptosis related cysteine protease; Caspase3; CPP32; CPP32B; Cysteine protease CPP32; Human cysteine protease CPP32 isoform alpha mRNA complete cds; PARP cleavage protease; SCA 1; SCA1; SREBP cleavage activity 1; Yama; CASP3_HUMAN; Caspase-3; CASP-3; Apopain; Protein Yama; SREBP cleavage activity 1; SCA-1.
产品应用	WB=1:500-2000, ELISA=1:5000-10000 Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
交叉反应	Human, Mouse, Rat
抗体来源	Rabbit
免疫原	KLH conjugated synthetic peptide derived from human caspase-3 p17 subunit
亚型	IgG
性状	Liquid
纯化方法	affinity purified by Protein A
克隆类型	Polyclonal
理论分子量	17/32 kDa
浓度	1mg/ml
储存液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究领域	Cancer > Cell Death > Apoptosis > Apoptosis Markers > Caspases Cancer > Cell Death > Apoptosis > Metabolism Cancer > Invasion/microenvironment > Apoptosis > Caspases Cell Biology > Apoptosis > Intracellular > Caspases etc > Caspases Cell Biology > Proteolysis / Ubiquitin > Proteolytic enzymes > Other proteases Metabolism > Pathways and Processes > Metabolism processes > Apoptosis
亚基	Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce.
亚细胞定位	Cytoplasm.
组织特异性	Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
翻译后修饰	Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa._x000D_ S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
相似性	Belongs to the peptidase C14A family.
功能	Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-\|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
背景资料	The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.

应用	推荐稀释比例
{WB}	{1:500-2000}
{ELISA}	{1:5000-10000}

Sample:
_x000D_ Lane 1: Raji (Human) Cell Lysate at 30 ug
_x000D_ Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug
_x000D_ Lane 3: Lung (Mouse) Lysate at 40 ug
_x000D_ Lane 4: Lung (Rat) Lysate at 40 ug
_x000D_ Primary: Anti-Caspase-3 (bs-0081R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 35 kD
_x000D_ Observed band size: 37 kD

Sample:
_x000D_ Lane 1: Spleen (Mouse) Lysate at 40 ug
_x000D_ Lane 2: Lung (Mouse) Lysate at 40 ug
_x000D_ Lane 3: Lymph node (Mouse) Lysate at 40 ug
_x000D_ Lane 4: Cerebrum (Mouse) Lysate at 40 ug
_x000D_ Lane 5: NIH/3T3 (Mouse) Cell Lysate at 30 ug
_x000D_ Lane 6: Spleen (Rat) Lysate at 40 ug
_x000D_ Lane 7: Lung (Rat) Lysate at 40 ug
_x000D_ Lane 8: Lymph node (Rat) Lysate at 40 ug
_x000D_ Lane 9: Cerebrum (Rat) Lysate at 40 ug
_x000D_ Lane 10: Hela (Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti-Caspase-3 (bs-0081R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 35 kD
_x000D_ Observed band size: 37 kD

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