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Caspase-3 Rabbit pAb(bs-0081R)

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  • ¥1180 - 1980
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  • bs-0081R
  • 2025年10月01日
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      50ul/100ul

    规格:50ul产品价格:¥1180.0
    规格:100ul产品价格:¥1980.0
    产品编号bs-0081R
    英文名称Caspase-3 Rabbit pAb
    中文名称半胱胺酸蛋白酶蛋白-3抗体
    英文别名Caspase-3 subunit p17; cleaved Caspase 3; cleaved Caspase-3; APOPAIN; CASP3; Caspase 3 apoptosis related cysteine protease; Caspase3; CPP32; CPP32B; Cysteine protease CPP32; Human cysteine protease CPP32 isoform alpha mRNA complete cds; PARP cleavage protease; SCA 1; SCA1; SREBP cleavage activity 1; Yama; CASP3_HUMAN; Caspase-3; CASP-3; Apopain; Protein Yama; SREBP cleavage activity 1; SCA-1.
    产品应用WB=1:500-2000, ELISA=1:5000-10000

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human caspase-3 p17 subunit
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量17/32 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Cell Death > Apoptosis > Apoptosis Markers > Caspases

    Cancer > Cell Death > Apoptosis > Metabolism

    Cancer > Invasion/microenvironment > Apoptosis > Caspases

    Cell Biology > Apoptosis > Intracellular > Caspases etc > Caspases

    Cell Biology > Proteolysis / Ubiquitin > Proteolytic enzymes > Other proteases

    Metabolism > Pathways and Processes > Metabolism processes > Apoptosis

    亚基Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce.
    亚细胞定位Cytoplasm.
    组织特异性Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
    翻译后修饰Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa._x000D_
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
    相似性Belongs to the peptidase C14A family.
    功能Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro enzyme that is processed in cells undergoing apoptosis by self proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kDa) and small (12kDa) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 Ser29 and Asp175 Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells). Caspase 3 is the predominant caspase involved in the cleavage of amyloid beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {ELISA}{1:5000-10000}

     

    产品细节图片1
    Sample:
    _x000D_ Lane 1: Raji (Human) Cell Lysate at 30 ug
    _x000D_ Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug
    _x000D_ Lane 3: Lung (Mouse) Lysate at 40 ug
    _x000D_ Lane 4: Lung (Rat) Lysate at 40 ug
    _x000D_ Primary: Anti-Caspase-3 (bs-0081R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 35 kD
    _x000D_ Observed band size: 37 kD
    产品细节图片2
    Sample:
    _x000D_ Lane 1: Spleen (Mouse) Lysate at 40 ug
    _x000D_ Lane 2: Lung (Mouse) Lysate at 40 ug
    _x000D_ Lane 3: Lymph node (Mouse) Lysate at 40 ug
    _x000D_ Lane 4: Cerebrum (Mouse) Lysate at 40 ug
    _x000D_ Lane 5: NIH/3T3 (Mouse) Cell Lysate at 30 ug
    _x000D_ Lane 6: Spleen (Rat) Lysate at 40 ug
    _x000D_ Lane 7: Lung (Rat) Lysate at 40 ug
    _x000D_ Lane 8: Lymph node (Rat) Lysate at 40 ug
    _x000D_ Lane 9: Cerebrum (Rat) Lysate at 40 ug
    _x000D_ Lane 10: Hela (Human) Cell Lysate at 30 ug
    _x000D_ Primary: Anti-Caspase-3 (bs-0081R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 35 kD
    _x000D_ Observed band size: 37 kD

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Bacterial microbots for acid-labile release of hybrid micelles to promote the synergistic antitumor efficacy.Acta Biomater. 2018 Sep 15;78:198-210. IHC-P, Mouse [IF=6.375]Zhou,et al.CXCR4 antagonist AMD3100 enhances the response of MDA-MB-231 triple-negative breast cancer cells to ionizing radiation.(2018) Cancer Letters. 418:196-203.IHC-P + WB, Mouse [IF=6.217]Wang L et al. Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/2 and Akt pathways. J Exp Clin Cancer Res. 2019 Feb 21;38(1):93.WB, Mouse [IF=6.01]Chen, Zhoujiang, et al. "Tunable conjugation densities of camptothecin on hyaluronic acid for tumor targeting and reduction-triggered release." Acta Biomaterialia (2016).IHC-P, Mouse [IF=5.47]Pan, Bo, et al. "c-Abl Tyrosine Kinase Mediates Neurotoxic Prion Peptide-Induced Neuronal Apoptosis via Regulating Mitochondrial Homeostasis." Molecular Neurobiology (2014): 1-15., Rat [IF=5.23]Zhao, Yong, et al. "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." Scientific Reports 6 (2016): 37884.WB, Pig [IF=5.168]Li, Bo, et al. "Long noncoding RNA CCAT1 functions as a ceRNA to antagonize the effect of miR-410 on the down-regulation of ITPKB in human HCT-116 and HCT-8 cells." Oncotarget 8.54 (2017): 92855.WB, Human [IF=5.154]Wu et al. Roles of Pannexin-1 Channels in Inflammatory Response through the TLRs/NF-Kappa B Signaling Pathway Following Experimental Subarachnoid Hemorrhage in Rats. (2017) Front.Mol.Neurosc. 10:175WB, Rat [IF=5.1]Wang, Caixia, et al. "Oral 4-(N)-stearoyl gemcitabine nanoparticles inhibit tumor growth in mouse models." Oncotarget 8.52 (2017): 89876-89886.IHC-P, Mouse [IF=5.047]Chen Z et al. Synergistic antitumor efficacy of hybrid micelles with mitochondrial targeting and stimuli-responsive drug release. Journal of Materials Chemistry B.2019.IHC-P, Human [IF=4.94]Kubatka, Peter, et al. "Antineoplastic effects of clove buds (Syzygium aromaticum L.) in the model of breast carcinoma." Journal of Cellular and Molecular Medicine (2017).IHC-P, Rat [IF=4.784]Zhu B et al. The hepatoprotective effect of polysaccharides from Pleurotus ostreatus on carbon tetrachloride-induced acute liver injury rats.Int J Biol Macromol. 2019 Jun 15;131:1-9. IHF, Rat [IF=4.75]Rosenzweig, Derek H., Sing J. Ou, and Thomas M. Quinn. ʺP38 mitogen‐activated protein kinase promotes dedifferentiation of primary articular chondrocytes in monolayer culture.ʺ Journal of Cellular and Molecular Medicine (2013)WB, Bovine [IF=4.527]Wan F et al. Long-term exposure to copper induces autophagy and apoptosis through oxidative stress in rat kidneys. Ecotoxicol Environ Saf. 2020 Mar 1;190:110158. WB, Rat [IF=4.525]Truffi,et al.Inhibition of Fibroblast Activation Protein Restores a Balanced Extracellular Matrix and Reduces Fibrosis in Crohn's Disease Strictures Ex Vivo.(2018) Inflammatory Bowel Diseases. 24:332-345.IF(IHC-F), Human [IF=4.522]Xiao L et al. Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression. J Cell Physiol. 2019 Jan 22. WB, Sheep [IF=4.38]Ma, Xiangyi, et al. "DT390-triTMTP1, a novel fusion protein of diphtheria toxin with tandem repeat TMTP1 peptide, preferentially targets metastatic tumors." Molecular pharmaceutics 10.1 (2013): 115-126.IHC-P, Human [IF=4.357]Li et al. Morusin suppresses breast cancer cell growth in vitro and in vivo through C/EBPβ and PPARγ mediated lipoapoptosis. (2015) J.Exp.Clin.Cancer.Res. 34:137WB, Human [IF=4.28]Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).IHC-P, Mouse [IF=4.26]Rosenzweig, Derek H., et al. "Mechanical injury of bovine cartilage explants induces depth-dependent, transient changes in MAP kinase activity associated with apoptosis." Osteoarthritis and Cartilage (2012).WB, Bovine [IF=4.254]Gao L et al. Restoration of E-cadherin by PPBICA protects against cisplatin-induced acute kidney injury by attenuating inflammation and programmed cell death. Lab Invest. 2018 Jul;98(7):911-923. WB, Mouse [IF=4.22]Sondag, Gregory R., et al. "Osteoactivin Induces Transdifferentiation of C2C12 Myoblasts into Osteoblasts." Journal of Cellular Physiology (2013)., Mouse [IF=4.2]Tian, A., et al. "Nanoscale TiO 2 nanotubes govern the biological behavior of human glioma and osteosarcoma cells." International Journal of Nanomedicine10 (2015): 2377-2389., Human [IF=4.12]Wang et al. Kukoamine A inhibits human glioblastoma cell growth and migration through apoptosis induction and epithelial-mesenchymal transition attenuation. (2016) Sci.Rep. 6:36543WB, Human [IF=3.974]Liao J et al. Inhibition of Caspase-1-dependent pyroptosis attenuates copper-induced apoptosis in chicken hepatocytes.Ecotoxicol Environ Saf. 2019 Jun 15;174:110-119.WB, Chicken [IF=3.95]Wang, Gang, et al. "Inhibition of hydrogen sulfide synthesis provides protection for severe acute pancreatitis rats via apoptosis pathway." Apoptosis (2013): 1-15.IHC-P, Rat [IF=3.923]Song LL et al. TGF-β and HSP70 profiles during transformation of yak hair follicles from the anagen to catagen stage. J Cell Physiol. 2019 Feb 5. WB, Yak [IF=3.92]Wei, Gang, et al. "β-Asarone inhibits neuronal apoptosis via the CaMKII/CREB/Bcl-2 signaling pathway in an in vitro model and AβPP/PS1 mice." Journal of Alzheimer's Disease 33.3 (2013): 863-880.IF(ICC), Mouse [IF=3.85]Wang, Yandi, et al. "Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis." Toxicology and Applied Pharmacology (2016).IHC-P, Sheep [IF=3.84]Kubatka, Peter, et al. "Fruit peel polyphenols demonstrate substantial anti-tumour effects in the model of breast cancer." European Journal of Nutrition(2015): 1-11.IHC-P, Rat [IF=3.83]Yang et al. MicroRNA-145 Increases the Apoptosis of Activated Hepatic Stellate Cells Induced by TRAIL through NF-κB Signaling Pathway. (2018) Front.Pharmacol. 8:980WB, Mouse [IF=3.82]Clark, Andrea J., et al. "WO3/Pt nanoparticles are NADPH oxidase biomimetics that mimic effector cells in vitro and in vivo." Nanotechnology27.6 (2015): 065101., [IF=3.79]Luo, Xiaoming, et al. "Antimetastasis and antitumor efficacy promoted by sequential release of vascular disrupting and chemotherapeutic agents from electrospun fibers." International Journal of Pharmaceutics (2014).IHC-P, Mouse [IF=3.743]Li Z et al.  Zinc oxide nanoparticles induce human multiple myeloma cell death via reactive oxygen species and Cyt-C/Apaf-1/Caspase-9/Caspase-3 signaling pathway in vitro. Biomed Pharmacother. 2020 Feb;122:109712.WB, Human [IF=3.743]Tan J et al. Integrative findings indicate anti-tumor biotargets and molecular mechanisms of calycosin against osteosarcoma. Biomed Pharmacother. 2020 Jun;126:110096.Immunostaining, human [IF=3.687]Kubatka P et al. Anticancer Activities of Thymus vulgaris L. in Experimental Breast Carcinoma in Vivo and in Vitro. Int J Mol Sci. 2019 Apr 9;20(7). pii: E1749.IHC-P, Rat&Mouse [IF=3.68]Xiao, Ke, et al. "The role of visfatin on the regulation of inflammation and apoptosis in the spleen of LPS-treated rats." Cell and Tissue Research (2014).IHC-P, Rat [IF=3.571]Chen S et al. Anthocyanins from Lycium ruthenicum Murr. ameliorated D-galactose-induced memory impairment, oxidative stress, and neuroinflammation in adult rats.J Agric Food Chem. 2019 Mar 20;67(11):3140-3149.WB&IHC-P, Rat [IF=3.53]Fang C, Zhang J, Qi D, Fan X, Luo J, et al. (2014) Evodiamine Induces G2/M Arrest and Apoptosis via Mitochondrial and Endoplasmic Reticulum Pathways in H446 and H1688 Human Small-Cell Lung Cancer Cells. PLoS ONE 9(12): e115204. WB, Human [IF=3.52]Chu et al. Newcastle Disease Virus V Protein Inhibits Cell Apoptosis and Promotes Viral Replication by Targeting CacyBP/SIP. (2018) Front.Cell.Infect.Microbiol. 8:304WB, [IF=3.499]Zhan C et al. Rotenone and 3-bromopyruvate toxicity impacts electrical and structural cardiac remodeling in rats. Toxicol Lett. 2019 Oct 1. pii: S0378-4274(19)30295-4. IHC-P, Rat [IF=3.47]Kubatka, Peter, et al. "Oregano demonstrates distinct tumour-suppressive effects in the breast carcinoma model." European Journal of Nutrition (2016): 1-14.IHC-P, Rat [IF=3.448]Xu B et al. Excessive mechanical stress induces chondrocyte apoptosis through TRPV4 in an anteriorcruciate ligament-transected rat osteoarthritis model. Life Sci. 2019 Jul 1;228:158-166. WB, Rat [IF=3.332]Muhammad T et al. Aloperine in combination with therapeutic adenoviral vector synergistically suppressed the growth of non-small cell lung cancer. J Cancer Res Clin Oncol. 2020 Feb 22. WB, Human [IF=3.329]Zhang Y et al. Overexpressed CES2 has prognostic value in CRC and knockdown CES2 reverses L-OHP-resistance in CRC cells by inhibition of the PI3K signaling pathway. Exp Cell Res. 2020 Jan 22:111856. WB, Human [IF=3.266]Chen S et al. Comparative assessment of the biocompatibility and degradation behavior of Zn-3Cu and JDBM alloys used for biliary surgery. Am J Transl Res. 2020 Jan 15;12(1):19-31. IHC-P, beagle [IF=3.23]Wang, Yu, et al. "Ibutilide treatment protects against ER stress induced apoptosis by regulating calumenin expression in tunicamycin treated cardiomyocytes." PloS one 12.4 (2017): e0173469.WB, Rat [IF=3.17]Liu, Yang, et al. "Amelioration of Stroke-Induced Neurological Deficiency by Lyophilized Powder of Catapol and Puerarin." International Journal of Biological Sciences 10.4 (2014): 448-456.WB, Mouse [IF=3.156]Andharia,et al.Electrophysiological properties of anion exchangers in the luminal membrane of guinea pig pancreatic duct cells.(2018) Pflugers Archiv: European Journal of Physiology. 470:897-907.IHC-P + WB, Guinea Pig [IF=3.146]Gao, Hui, et al. "Hispidulin induces mitochondrial apoptosis in acute myeloid leukemia cells by targeting extracellular matrix metalloproteinase inducer."American Journal of Translational Research 8.2 (2016): 1115-1132WB, Human [IF=3.138]Chen J et al. Effect of artemisinin on proliferation and apoptosis-related protein expression in vivo and in vitro.Saudi J Biol Sci. 2018 Nov;25(7):1488-1493.WB&IHC, Rat [IF=3.13]Zhang, Wen-feng, et al. "Angelica polysaccharides inhibit the growth and promote the apoptosis of U251 glioma cells in vitro and in vivo." Phytomedicine (2017).WB, Human [IF=3.105]Yang F et al. Copper induces oxidative stress and apoptosis through mitochondria-mediated pathway in chicken hepatocytes.(2019)Toxicol In Vitro. Feb;54:310-316. WB, Chicken [IF=3.063]Liu T et al. MicroRNA-193b-3p regulates hepatocyte apoptosis in selenium-deficient broilers by targeting MAML1.J Inorg Biochem. 2018 Sep;186:235-245. WB, broiler chick [IF=3.06]Li, Guiying, et al. ʺGremlin Aggravates Hyperglycemia‐Induced Podocyte Injury by a TGFβ/Smad Dependent Signaling Pathway.ʺ Journal of CellularBiochemistry (2013).WB, Mouse [IF=3.06]Yan Y et al. Inhibition of TGF-β Signaling in Gliomas by the Flavonoid Diosmetin Isolated from Dracocephalum peregrinum L. Molecules. 2020 Jan 2;25(1). pii: E192. WB, Human [IF=3.043]Chen Y et al. Conditional deletion of Bmp2 in cranial neural crest cells recapitulates Pierre Robin sequence in mice.(2018)Cell Tissue Res.Nov 9.IHC, Mouse [IF=3.01]Wang Y et al. Effects of Circadian Rhythm Disorder on the Hippocampus of SHR and WKY Rats. Neurobiol Learn Mem. 2019 Dec 16;168:107141.IHC-P, Rat
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      Caspase-3 Rabbit pAb(bs-0081R)-50ul/100ul
      ¥1180 - 1980