- ￥1300
- AntibodySystem
- RGK26101
- 2025年07月30日
- ELISA, WB
- Mouse
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- 详细信息
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- 文献和实验
- 技术资料
- 抗体名：
Anti-Strep Tag (WSHPQFEK) Antibody (C23.21)
- 抗体英文名：
Anti-Strep Tag (WSHPQFEK) Antibody (C23.21)
- 浓度：
0.56 mg/ml
- 应用范围：
ELISA, WB
- 宿主：
Mouse
- 保质期：
1 year
- 级别：
科研级
- 库存：
999
- 供应商：
AntibodySystem
- 克隆性：
Monoclonal
- 亚型：
IgG1, kappa
- 规格：
100ug
| 宿主 | Mouse |
| 经测试应用 | ELISA: 1:2000-1:8000, WB: 1:500-1:5000 |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS, pH 7.4. |
| 浓度 | 0.56 mg/ml |
| 纯度 | >95% as determined by SDS-PAGE. |
| 克隆类型 | Monoclonal |
| 同种型 | IgG1, kappa |
| 应用 | ELISA, WB |
| 靶标 | Strep Tag (WSHPQFEK), Strep-Tag, Strep-Tag II peptide (WSHPQFEK),streptag,StrepTag II |
| 纯化方式 | Protein A/G purified from cell culture supernatant. |
| 内毒素水平 | Please contact with the lab for this information. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| 克隆号 | C23.21 |
| 备注 | For research use only. |
AntibodySystem Laboratories SAS was established in 2019 in France as an independent brand entity focused on the research, development, and production of protein and antibody reagents in the life sciences field. Our mission is to provide high-quality protein and antibody products to life science researchers worldwide. AntibodySystem's extensive product range includes traditional polyclonal antibodies, classic housekeeping antibodies, tag antibodies, high-activity proteins, in vivo functional antibodies, low-background flow cytometry antibodies, high-specificity nanobodies, specialized small molecule antibodies, premium phospho-specific antibodies, DNA/RNA antibodies, PEG antibodies, and more—spanning over ten product series.
Currently, AntibodySystem's product lines cover multiple research areas, including 48 types of viruses and superbugs, parasites, tumors, Alzheimer's disease, Parkinson's disease, hypersensitivity and allergic reactions, immune suppression, and immune activation.
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文献和实验期刊信息：Nature communications vol. 16,1 3852. 24 Apr. 2025
作者： Tang, Mingqing et al.
标题：A facultative plasminogen-independent thrombolytic enzyme from Sipunculus nudus
IF: 14.7
-His-Pro-Gln-Phe-Glu-Lys) 可放在N- 或 C-terminus 不影响蛋白折叠和功能，无需移除 分子量 1058Da Twin-Strep-tag® （也有叫做双Strep-tag®II） 28 aa 标签(WSHPQFEK-GGGSGGGSGG-SA-WSHPQFEK) 两段Strep-tag®II标签序列 较Strep-tag®II对于Strep-Tactin®有更高亲和力 分子量 2887Da 二者均为小标签，不会对蛋白质的结构和功能活性产生影响，因此通常不需要在纯化
，在中性和弱碱性条件下可以和固定化的金属离子相互作用(如Ni离子，Zn离子，Co离子等)，从而达到纯化蛋白的目的。此系统的洗脱方式可以用过三种非特异性步骤达成： 降低pH（4.5-6） b. 加入EDTA C.不同浓度咪唑溶液（20-250mM） 当蛋白洗脱完毕后，使用EDTA使纯化树脂与金属离子分离，即可完成树脂的再生。 Strep-tag®则是基于链霉亲和素(Streptavidin)和生物素这一自然的相互作用，将Strep-tag®II（WSHPQFEK）或Twin-Strep-tag®
Purification and Analysis of Strep-tagged Antibody-Fragments
The Strep-tag is a nine-residue minimal peptide sequence with intrinsic affinity towards streptavidin. This peptide – or its improved version Strep-tag II – can be fused to recombinant antibody fragments and other proteins in various fashions
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