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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温运输，-20℃保存
- 保质期：
1年
- 英文名：
pINT3
- 库存：
大量
- 供应商：
信裕生物
质粒编号:
质粒名称: pINT3
其他名称:
质粒大小（bp）: 5694
抗性: Ampicillin
质粒类型: Integrating vector
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平: High Copy
蛋白标签:
测序引物:
GenBank号:
其他属性: Gentamicin
质粒图谱:
质粒序列:
>pINT3 sequence 5694 bps
gatcctctagagtcgacctgcaggcatgcaagcttgcatgcctgcaggtcgactctagaggatccccggg
taccgagctcgaattggccgcggcgttgtgacaatttaccgaacaactccgcggccgggaagccgatctc
ggcttgaacgaattgttaggtggcggtacttgggtcgatatcaaagtgcatcacttcttcccgtatgccc
aactttgtatagagagccactgcgggatcgtcaccgtaatctgcttgcacgtagatcacataagcaccaa
gcgcgttggcctcatgcttgaggagattgatgagcgcggtggcaatgccctgcctccggtgctcgccgga
gactgcgagatcatagatatagatctcactacgcggctgctcaaacctgggcagaacgtaagccgcgaga
gcgccaacaaccgcttcttggtcgaaggcagcaagcgcgatgaatgtcttactacggagcaagttcccga
ggtaatcggagtccggctgatgttgggagtaggtggctacgtctccgaactcacgaccgaaaagatcaag
agcagcccgcatggatttgacttggtcagggccgagcctacatgtgcgaatgatgcccatacttgagcca
cctaactttgttttagggcgactgccctgctgcgtaacatcgttgctgctgcgtaacatcgttgctgctc
cataacatcaaacatcgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgtacaa
aaaaacagtcataacaagccatgaaaaccgccactgcgccgttaccaccgctgcgttcggtcaaggttct
ggaccagttgcgtgagcgcatacgctacttgcattacagtttacgaaccgaacaggcttatgtcaattcg
agctcggtacccggggatcctctagagtcgaccaccaagggcaccatctctgcttgggccaccccgttgg
ccgcagccagctcgctgagagccgtgaacgacagggcgaacgccagcccgccgacggcgagggttccgac
cgctgcaactcccggtgcaaccttgtcccggtctattctcttcactgcaccagctccaatctggtgtgaa
tgcccctcgtctgttcgcgcaggcggggggctctattcgtttgtcagcatcgaaagtagccagatcaggg
atgcgttgcaaccgcgtatgcccaggtcagaagagtcgcacaagagttgcagacccctggaaagaaaaat
ggccagagggcgaaaacaccctctgaccagcggagcgggcgacgggaatcgaacccgcgtagctagtttg
gaagaatgggtgtctgccgaccacatatgggccggtcaagataggtttttaccccctctcggctgcatcc
tctaagtggaaagaaattgcaggtcgtagaagcgcgttgaagcctgagagttgcacaggagttgcaaccc
ggtagccttgttcacgacgagaggagacctagttggcacgtcgcggatggggatcgctgaagactcagcg
cagcgggaggatccaagcctcatacgtcaacccgcaggacggtgtgaggtactacgcgctgcagacctac
gacaacaagatggacgccgaagcctggctcgcgggcgagaagcggctcatcgagatggagacctggaccc
ctccacaggaccgggcgaagaaggcagccgccagcgccatcacgctggaggagtacacccggaagtggct
cgtggagcgcgacctcgcagacggcaccagggatctgtacagcgggcacgcggagcgccgcatctacccg
gtgctaggtgaagtggcggtcacagagatgacgccagctctggtgcgtgcgtggtgggccgggatgggta
ggaagcacccgactgcccgccggcatgcctacaacgtcctccgggcggtgatgaacacagcggtcgagga
caagctgatcgcagagaacccgtgccggatcgagcagaaggcagccgatgagcgcgacgtagaggcgctg
acgcctgaggagctggacatcgtcgccgctgagatcttcgagcactaccggatcgcggcatacatcctgg
cgtggacgagcctccggttcggagagctgatcgagcttcgccgcaaggacatcgtggacgacggcatgac
gatgaagctccgggtgcgccgtggcgcttcccgcgtggggaacaagatcgtcgttggcaacgccaagacc
gtccggtcgaagcgtcctgtgacggttccgcctcacgtcgcggagatgatccgagcgcacatgaaggacc
gtacgaagatgaacaagggccccgaggcattcctggtgaccacgacgcagggcaaccggctgtcgaagtc
cgcgttcaccaagtcgctgaagcgtggctacgccaagatcggtcggccggaactccgcatccacgacctc
cgcgctgtcggcgctacgttcgccgctcaggcaggtgcgacgaccaaggagctgatggcccgtctcggtc
acacgactcctaggatggcgatgaagtaccagatggcgtctgaggcccgcgacgaggctatcgctgaggc
gatgtccaagctggccaagacctcctgaaacgcaaaaagcccccctcccaaggacactgagtcctaaaga
ggggggtttcttgtcagtacgcgaagaaccacgcctggccgcgagcgccagcaccgccgctctgtgcgga
gacctgggcaccagccccgccgccgccaggagcattgccgttcccgccagctgagttctgttgtgcgccg
cctatgtagagctggtcgttgtaggtccgatctccaggcgactttccggcgacgctgaggatgtcgatca
cagagcctccgggaccgccggttgcggtcaaacctgaccatccgacagcggacgccgtggtgtttcctcc
agggcctccggccttgcctgagaatacagagccagctcccgctgcgcctccagctccgacgagcccggtg
atcgtcttggtcgacctgcaggcatgcaagcttggcactggccgtcgttttacaacgtcgtgactgggaa
aaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaag
aggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattt
tctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgcc
gcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccgg
catccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcacc
gaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtt
tcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatac
attcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagag
tatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgct
cacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaac
tggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttt
taaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcata
cactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacag
taagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgat
cggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgg
gaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaa
cgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatgga
ggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatct
ggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcg
tagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgc
ctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaactt
catttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtg
agttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttct
gcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagag
ctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgt
agccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgtt
accagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggat
aaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccg
aactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggta
tccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctt
tatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcgga
gcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacat
gttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgct
cgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaac
cgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcggg
cagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgctt
ccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgatt
acgaattcgagctcggtacccggg
>24778 with M13pUC-fwd
GAGTCGACCTGCAGGCATGCAAGCTTTGCATGCCTGCAGGTCNACCAAGACGATCACCGGGCTCGTCGGA
GCTGGAGGCGCAGCGGGAGCTGGCTCTGTATTCTCAGGCAAGGCCGGAGGCCCTGGAGGAAACACCACGG
CGTCCGCTGTCGGATGGTCAGGTTTGACCGCAACCGGCGGTCCCGGAGGCTCTGTGATCGACATCCTCAG
CGTCGCCGGAAAGTCGCCTGGAGATCGGACCTACAACGACCAGCTCTACATAGGCGGCGCACAACAGAAC
TCAGCTGGCGGGAACGGCAATGCTCCTGGCGGCGGCGGGGCTGGTGCCCAGGTCTCCGCACAGAGCGGCG
GTGCTGGCGCTCGCGGCCAGGCGTGGTTCTTCGCGTACTGACAAGAAACCCCCCTCTTTAGGACTCAGTG
TCCTTGGGAGGGGGGCTTTTTGCGTTTCAGGAGGTCTTGGCCAGCTTGGACATCGCCTCAGCGATAGCCT
CGTCGCGGGCCTCAGACGCCATCTGGTACTTCATCGCCATCCTAGGAGTCGTGTGACCGAGACGGGCCAT
CAGCTCCTTGGTCGTCGCACCTGCCTGAGCGGCGAACGTAGCGCCGACAGCGCGGAGGTCGTGGATGCGG
AGTTCCGGCCGACCGATCTTGGCGTAGCCACGCTTCAGCGACTTGGTGAACGCGGACTTCGACAGCCGGT
TGCCCTGCGTCGTGGTCACCAGGAATGCCTCGGGGCCCTTGTTCATCTTCGTACGGTCCTTCATGTGCGC
TCGGATCATCTCCGCGACGTGAGGCGGAACCGTCACAGGACGCTTCGACCGGACGGTCTTGGCGTTGCNA
ACGAC
>24778 with M13rev
ACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTGGCCGCGGCGTTGTGACAATTTACCGAACAACTCCG
CGGCCGGGAAGCCGATCTCGGCTTGAACGAATTGTTAGGTGGCGGTACTTGGGTCGATATCAAAGTGCAT
CACTTCTTCCCGTATGCCCAACTTTGTATAGAGAGCCACTGCGGGATCGTCACCGTAATCTGCTTGCACG
TAGATCACATAAGCACCAAGCGCGTTGGCCTCATGCTTGAGGAGATTGATGAGCGCGGTGGCAATGCCCT
GCCTCCGGTGCTCGCCGGAGACTGCGAGATCATAGATATAGATCTCACTACGCGGCTGCTCAAACTTGGG
CAGAACGTAAGCCGCGAGAGCGCCAACAACCGCTTCTTGGTCGAAGGCAGCAAGCGCGATGAATGTCTTA
CTACGGAGCAAGTTCCCGAGGTAATCGGAGTCCGGCTGATGTTGGGAGTAGGTGGCTACGTCTCCGAACT
CACGACCGAAAAGATCAAGAGCAGCCCGCATGGATTTGACTTGGTCAGGGCCGAGCCTACATGTGCGAAT
GATGCCCATACTTGAGCCACCTAACTTTGTTTTAGGGCGACTGCCCTGCTGCGTAACATCGTTGCTGCTG
CGTAACATCGTTGCTGCTCCATAACATCAAACATCGACCCACGGCGTAACGCGCTTGCTGCTTGGATGCC
CGAGGCATAGACTGTACAAAAAAACAGTCATAACAAGCCATGAAAACCGCCACTGCGCCGTTACCACCGC
TGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTGCATTACAGTTTACGAACCGA
ACAGGCTTATGTCAATTCGAGCTCGG
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文献和实验a small amunt f tnsils at a time. (At this pint yu can freeze the tnsils until yu have enugh) 6.Set up the hmgenizer with the large prbe (1cm diameter). 7.Put a small amunt f minced tnsil in the 50ml Crning tube and add lysis buffer t ~10ml mark
Basic Methods of Culturing Drosophila
from cultures established with an optimum number of animals. Expect 50-100 adults from a vigorous 8 dram vial culture, 300-600 from a comparable half-pint bottle culture. For most genotypes the optimum number of females will range from 1 to 3 per vial
Basic Methods of Culturing Drosophila
and liquified medium. The best yield of healthy adults is obtained from cultures established with an optimum number of animals. Expect 50-100 adults from a vigorous 8 dram vial culture, 300-600 from a comparable half-pint bottle culture. For most genotypes
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