- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4℃保存
- 保质期:
6个月
- 英文名:
EpiQuik Dnmt3B Assay Kit
- 库存:
1
- 供应商:
上海信裕
- Dnmt3B 分析试剂盒详细信息:
-
Product Overview
The EpiQuik™ Dnmt3B Assay Kit is a convenient package of tools that allows the experimenter to measure the amount of Dnmt3B at tremendously fast speeds and consistency, superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful Dnmt3B assay experiment. The EpiQuik™ Dnmt3B Assay Kit is suitable for measuring Dnmt amounts quantitatively from fresh tissue and cultured cells of human and mouse.
WHY CHOOSE THE EPIQUIK™ DNMT3B ASSAY KIT?
- The fastest procedure, which can be finished within 3 hours.
- Innovative colorimetric assay, which quantitatively measure Dnmt3B amount with no need for electrophoresis.
- Strip microplate format makes the assay flexible: manual or high throughput analysis.
- Simple, reliable, and consistent assay conditions.
Dnmt3B 分析试剂盒Principle & Procedure
The EpiQuik™ Dnmt3B Assay Kit is designed for measuring total Dnmt3B amount from tissues or cells. In an assay with this kit, the unique Dnmt affinity substrate is stably coated on the strip well.. The sample is added into the well and Dnmt3B contained in the sample binds to the substrate. The bound Dnmt3B can be recognized with specific Dnmt3B antibody and colorimetrically quantified through an ELISA-like reaction. The amount of Dnmt3B is proportional to the intensity of color development.
SCHEMATIC PROCEDURE:
Dnmt3B 分析试剂盒Product Components
DB1 (10x wash buffer)
DB2 (assay buffer)
DB3 (Dnmt3B standard, 20 µg/ml)*
DB4 (affinity antibody 100 µg/ml)*
DB5 (detection antibody 200 µg/ml)*
DB6 (developing solution)
DB7 (stop solution)
8 well assay strips (with frame)
User guide
xy105 山羊血清
xy106 绵羊血清
xy107 鸡血清
xy108 驴血清
xy109 猪血清
xy110 胎牛血清(澳洲血源)
xy111 特级胎牛血清
xy112 优级胎牛血清
xy113 标准胎牛血清
xy114 优级新生牛血清
xy115 标准新生牛血清
xy116 成牛血清
xy117 特级马血清
xy118 标准马血清
xy119 脱纤维绵羊血
xy120 牛血清白蛋白V
xy121 牛血清白蛋白全
xy201 RNA上样缓冲液(6×)
xy202 DNA上样缓冲液(6×Ⅳ)
xy203 Zoman-Green (EB升级换代产品)
xy204 EB/溴化乙锭(10mg/ml)
xy205-1 TAE电泳缓冲液(50×)
xy205-2 TAE电泳缓冲液(50×)
xy205-3 TAE电泳缓冲液(50×)
xy206 TBE电泳缓冲液(5×)
xy207 TAE电泳缓冲液RNA专用(50×)
xy301 蛋白标准(5mg/ml BSA)
xy303 PMSF(100mM)
xy304 5×tris-甘氨酸电泳缓冲液
xy305-1 SDS-PAGE电泳液(10X)
xy306 SDS-PAGE蛋白上样缓冲液(5X)
xy307 非变性PAGE蛋白上样缓冲液(5X)
xy309 10%过硫酸铵
xy310 30% 丙烯酰胺 (29:1)
* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.Dnmt3B 分析试剂盒User Guide & MSDS
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