pHG165

名称	编号	规格	价格	手册
pHG165

质粒编号:
质粒名称: pHG165
其他名称:
质粒大小（bp）: 3375
抗性: Ampicillin
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性: Created by Moore, July 1995, under contract with NCBI. Enzymes that cut only once (not including polylinker): AatII, AvaI, BalI, ScaI. The HaeII sites flanking lac in pUC8 were changed to HpaI sites. pBR322 was modified by removing PstI & HincII sites in ampR gene and changing the EcoRI to HpaI. A HpaI cassette with lac & MCS was cloned at the HpaI of the modified pBR322. pHG165 enables selection for inserts on MacConkey agar. The lac gene and multiple cloning sites are easily removed with a HpaI digest. Plasmid vector with the lac gene and polylinker of pUC8 but with copy number control of pBR322. Restriction digests of the clone give the following sizes (kb): HindIII–3.5; HpaI–3.0, about 0.5. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli JM101, E.coli, E.coli JM series. Related vectors: pBR322, pUC8.
质粒图谱:

质粒序列:

1	tcgggcagcg	ttgggtcctg	gccacgggtg	cgcatgatcg	tgctcctgtc	50
51	gttgaggacc	cggctaggct	ggcggggttg	ccttactggt	tagcagaatg	100
101	aatcaccgat	acgcgagcga	acgtgaagcg	actgctgctg	caaaacgtct	150
151	gcgacctgag	caacaacatg	aatggtcttc	ggtttccgtg	tttcgtaaag	200
201	tctggaaacg	cggaagtcag	cgccctgcac	cattatgttc	cggatctgca	250
251	tcgcaggatg	ctgctggcta	ccctgtggaa	cacctacatc	tgtattaacg	300
301	aagcgctggc	attgaccctg	agtgattttt	ctctggtccc	gccgcatcca	350
351	taccgccagt	tgtttaccct	cacaacgttc	cagtaaccgg	gcatgttcat	400
401	catcagtaac	ccgtatcgtg	agcatcctct	ctcgtttcat	cggtatcatt	450
451	acccccatga	acagaaatcc	cccttacacg	gaggcatcag	tgaccaaaca	500
501	ggaaaaaacc	gcccttaaca	tggcccgctt	tatcagaagc	cagacattaa	550
551	cgcttctgga	gaaactcaac	gagctggacg	cggatgaaca	ggcagacatc	600
601	tgtgaatcgc	ttcacgacca	cgctgatgag	ctttaccgca	gctgcctcgc	650
651	gcgtttcggt	gatgacggtg	aaaacctctg	acacatgcag	ctcccggaga	700
701	cggtcacagc	ttgtctgtaa	gcggatgccg	ggagcagaca	agcccgtcag	750
751	ggcgcgtcag	cgggtgttgg	cgggtgtcgg	ggcgcagcca	tgacccagtc	800
801	acgtagcgat	agcggagtgt	atactggctt	aactatgcgg	catcagagca	850
851	gattgtactg	agagtgcacc	atatgcggtg	tgaaataccg	cacagatgcg	900
901	taaggagaaa	ataccgcatc	aggcgctctt	ccgcttcctc	gctcactgac	950
951	tcgctgcgct	cggtcgttcg	gctgcggcga	gcggtatcag	ctcactcaaa	1000
1001	ggcggtaata	cggttatcca	cagaatcagg	ggataacgca	ggaaagaaca	1050
1051	tgtgagcaaa	aggccagcaa	aaggccagga	accgtaaaaa	ggccgcgttg	1100
1101	ctggcgtttt	tccataggct	ccgcccccct	gacgagcatc	acaaaaatcg	1150
1151	acgctcaagt	cagaggtggc	gaaacccgac	aggactataa	agataccagg	1200
1201	cgtttccccc	tggaagctcc	ctcgtgcgct	ctcctgttcc	gaccctgccg	1250
1251	cttaccggat	acctgtccgc	ctttctccct	tcgggaagcg	tggcgctttc	1300
1301	tcatagctca	cgctgtaggt	atctcagttc	ggtgtaggtc	gttcgctcca	1350
1351	agctgggctg	tgtgcacgaa	ccccccgttc	agcccgaccg	ctgcgcctta	1400
1401	tccggtaact	atcgtcttga	gtccaacccg	gtaagacacg	acttatcgcc	1450
1451	actggcagca	gccactggta	acaggattag	cagagcgagg	tatgtaggcg	1500
1501	gtgctacaga	gttcttgaag	tggtggccta	actacggcta	cactagaagg	1550
1551	acagtatttg	gtatctgcgc	tctgctgaag	ccagttacct	tcggaaaaag	1600
1601	agttggtagc	tcttgatccg	gcaaacaaac	caccgctggt	agcggtggtt	1650
1651	tttttgtttg	caagcagcag	attacgcgca	gaaaaaaagg	atctcaagaa	1700
1701	gatcctttga	tcttttctac	ggggtctgac	gctcagtgga	acgaaaactc	1750
1751	acgttaaggg	attttggtca	tgagattatc	aaaaaggatc	ttcacctaga	1800
1801	tccttttaaa	ttaaaaatga	agttttaaat	caatctaaag	tatatatgag	1850
1851	taaacttggt	ctgacagtta	ccaatgctta	atcagtgagg	cacctatctc	1900
1901	agcgatctgt	ctatttcgtt	catccatagt	tgcctgactc	cccgtcgtgt	1950
1951	agataactac	gatacgggag	ggcttaccat	ctggccccag	tgctgcaatg	2000
2001	ataccgcgag	acccacgctc	accggctcca	gatttatcag	caataaacca	2050
2051	gccagccgga	agggggccga	gcgcagaagt	ggtcctgcaa	ctttatccgc	2100
2101	ctccatccag	tctattaatt	gttgccggga	agctagagta	agtagttcgc	2150
2151	cagttaatag	tttgcgcaac	gttgttgcca	ttgctacagg	catcgtggtg	2200
2201	tcacgctcgt	cgtttggtat	ggcttcattc	agctccggtt	cccaacgatc	2250
2251	aaggcgagtt	acatgatccc	ccatgttgtg	caaaaaagcg	gttagctcct	2300
2301	tcggtcctcc	gatcgttgtc	agaagtaagt	tggccgcagt	gttatcactc	2350
2351	atggttatgg	cagcactgca	taattctctt	actgtcatgc	catccgtaag	2400
2401	atgcttttct	gtgactggtg	agtactcaac	caagtcattc	tgagaatagt	2450
2451	gtatgcggcg	accgagttgc	tcttgcccgg	cgtcaatacg	ggataatacc	2500
2501	gcgccacata	gcagaacttt	aaaagtgctc	atcattggaa	aattcttcgg	2550
2551	ggcgaaaact	ctcaaggatc	ttaccgctgt	tgagatccag	ttcgatgtaa	2600
2601	cccactcgtg	cacccaactg	atcttcagca	tcttttactt	tcaccagcgt	2650
2651	ttctgggtga	gcaaaaacag	gaaggcaaaa	tgccgcaaaa	aagggaataa	2700
2701	gggcgacacg	gaaatgttga	atactcatac	tcttcctttt	tcaatattat	2750
2751	tgaagcattt	atcagggtta	ttgtctcatg	agcggataca	tatttgaatg	2800
2801	tatttagaaa	aataaacaaa	taggggttcc	gcgcacattt	ccccgaaaag	2850
2851	tgccacctga	cgtctaagaa	accattatta	tcatgacatt	aacctataaa	2900
2901	aataggcgta	tcacgaggcc	ctttcgtctt	caagaattgt	taacgcgccc	2950
2951	aatacgcaaa	ccgcctctcc	ccgcgcgttg	gccgattcat	taatgcagct	3000
3001	ggcacgacag	gtttcccgac	tggaaagcgg	gcagtgagcg	caacgcaatt	3050
3051	aatgtgagtt	agctcactca	ttaggcaccc	caggctttac	actttatgct	3100
3101	tccggctcgt	atgttgtgtg	gaattgtgag	cggataacaa	tttcacacag	3150
3151	gaaacagcta	tgaccatgat	tacgaattcc	cggggatccg	tcgacctgca	3200
3201	gccaagcttg	gcactggccg	tcgttttaca	acgtcgtgac	tgggaaaacc	3giraffe-bp-2831250
3251	ctggcgttac	ccaacttaat	cgccttgcag	cacatccccc	tttcgccagc	3300
3301	tggcgtaata	gcgaagaggc	ccgcaccgat	cgcccttccc	aacagttgcg	3350
3351	cagcctgaat	ggcgaatggg	ttaac	3375