pBP81

Constructed by replacing the telomere-adjacent sequence from pBP63A with a 0.25 kb SalI/SphI fragment containing the YTCA-1x telomere and CEN6 sequences. [1] Linearize with SalI (site adjacent to the telomere sequence) before transformation. [1] Contains 2 head to tail Alu sequences with the 5′ end closer to the SalI site. [1] Centric chromosome fragmentation vector targeting Alu sequences. [1] Restriction digests of the clone give the following sizes (kb): BamHI–5.6, 0.35, 0.25; PstI–4.4, 1.4, 0.4; SstI–5.4, 0.8; SalI/SphI–6.2. (ATCC staff) The order of the major features in this plasmid is: bla – HIS3 – 3’Alu5′ – 3’Alu5′ – SalI – TEL – CEN6. [1] Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli, Saccharomyces cerevisiae YPH252, Saccharomyces cerevisiae CGY2516 [ATCC 74013].