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- 2025年07月14日
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- 文献和实验
- 技术资料
- 保存条件：
低温运输，-20℃保存
- 保质期：
1年
- 英文名：
lambda N- cI857 r32(lambdaNcI857r32)
- 库存：
大量
- 供应商：
信裕生物
质粒编号:
质粒名称: lambda N- cI857 r32
其他名称:
质粒大小（bp）:
抗性:
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性:
| Notes: |
This was isolated as spontaneous mutant of lambda papa prophage. It contains an IS2 terminator between the tR1 site and the O gene. This replicates in sup hosts (clear plaques at 42C; turbid plaques at 30C). It does not grow on sup0 hosts and does not kill a sup0 host when lysogenized and induced at 42C. (personal communication) This is bacteriophage lambda with mutant O and P genes regulated by the N product (functional only when N is supplied). It produces immunity to lambda. Genotype:Nam7 Nam53 cI857 r32 IS2 Medium is 1592 SM buffer. Hosts: bacteria-free lysate, E.coli, E.coli C600.
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质粒图谱:
质粒序列:
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文献和实验Screening lambda cDNA or genomic libraries
ZAP II.For lambda gt11 use the host Y1090.The Barstead mixed stage C.elegans cDNA library is in the original lamda ZAP vector,and requires a host carrying the supF mutation,such as Y1090.Growing the cells:grow up 40 ml of bacteria o/n in LBspin 5 min
µl (50µl) of lambda phage DNA/HindIII fragments (10µg/ml).7.Non-radioactive Labeling Mix (minus DIG-dUTP):50µl (150µl) of 1mM dGTP, 1mM dATP, 1mM dCTP, 0.65mM dTTP aqueous solution.8.Deionized Water: 1.5ml of water deionized on a Milli-Q®
SOUTHERN BLOTTING OF GENOMIC DNA
1.Digest DNA: • 1.5 μg DNA in a 25 μI reaction containing BSA • 3 to 5 units for 5 to 3 hours (aim for 10-fold overdigestion; remember many enzymes will not survive well for more than 3 hr). • Also prepare molecular weight standard (Lambda
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