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- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温运输，-20℃保存
- 保质期：
1年
- 英文名：
lambda EMBL4(lambdaEMBL4)
- 库存：
大量
- 供应商：
信裕生物
质粒编号:
质粒名称: lambda EMBL4
其他名称:
质粒大小（bp）: 43000
抗性:
质粒类型: Unspecified
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平:
蛋白标签:
测序引物:
GenBank号: X58667, X58668, X06780
其他属性: EMBL4 size is between 43000 and 44000 bp. left arm is 19300. right arm is 9200 bp. The stuffer is 1400 bp. Phage with inserts have the Spi- phenotype. [3] lambdaEMBL3 (ATCC 37266) and lambdaEMBL4 (ATCC 37267) differ only in the orientation of the MCS surrounding the stuffer fragment. Medium is 1592 SM buffer. Hosts: E.coli LE392, E.coli O358, E.coli O359, E.coli K802, E.coli NM538, E.coli NM539, E.coli KW251, E.coli XL-1-Blue MRA, E.coli XL-1-Blue MRA P2. Related vectors: lambda 1059, EMBL3.
质粒图谱:
质粒序列:
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文献和实验(now with the Department of Biology, Syracuse University), but I've added a few modifications (i.e. helpful hints) that I've found in various sources. 1.Grow a fresh overnight culture of lambda host cells (we use K803 for EMBL3) in LB or T-broth fortified with 10mM Mg
Genomic Cloning Technical Manual
, 2) the genomic library constructed should be representative of genomic DNA sequences, and 3) the strategy should facilitate restriction mapping of the cloned DNA. Promega's preparations of LambdaGEM ® -11 and EMBL Vector Arms are optimized for the highest
-HCl pH 7.5, 0.1 M NaCl, 10 mM MgCl2, 0.05 % gelatine; store over CHCl3. 2 M Tris-HCl pH 7.5 1.0 ml; 5 M NaCl 4.0 ml; 1 M MgCl2 2.0 ml; 0.5 % gelatine 20.0 ml; H2O 173.0 ml; Total 200.0 ml. Remarks: *: this value holds true for genomic phages (EMBL
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