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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温运输，-20℃保存
- 保质期：
1年
- 英文名：
pHIV-dTomato
- 库存：
大量
- 供应商：
信裕生物
质粒编号:
质粒名称: pHIV-dTomato
其他名称:
质粒大小（bp）: 7683
抗性: ampicillin
质粒类型: Mammalian Expression, Lentiviral
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平:
蛋白标签:
测序引物:
GenBank号:
其他属性:
质粒图谱:
质粒序列:
>Author sequence
gtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcata
gttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagct
acaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttc
gcgatgtacgggccagatatacgcgttgacattgattattgactagttattaatagtaatcaattacggg
gtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctga
ccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactt
tccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatat
gccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgacc
ttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggtttt
ggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgt
caatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattg
acgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagcgcgttttgcctgtactgggtct
ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaat
aaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccct
cagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaaggga
aaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcg
actggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagt
attaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata
aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaac
atcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttaga
tcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaag
ctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccggccgcgctga
tcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtaaa
aattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtg
ggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgc
tgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattga
ggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtg
gaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactg
ctgtgccttggaatgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatgga
gtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaa
gaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaa
attggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgc
tgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaacc
ccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattc
gattagtgaacggatcggcactgcgtgcgccaattctgcagacaaatggcagtattcatccacaatttta
aaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacataca
aactaaagaattacaaaaacaaattacaaaaattcaaaattttcgggtttattacagggacagcagagat
ccagtttggttagtaccgggcccgctctagccgtgaggctccggtgcccgtcagtgggcagagcgcacat
cgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcgg
ggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatata
agtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgt
gtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacctggct
gcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaa
ggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggt
ggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgc
gacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggttttt
ggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgc
ggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgcc
gtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccg
cttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcac
ccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgcc
gtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttat
gcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattct
ccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagttt
ttttcttccatttcaggtgtcgtgagcggccgctgaactgaattcatcgacgttaactattctagagtac
ccgggctaggatccgcccctctccctcccccccccctaacgttactggccgaagccgcttggaataaggc
cggtgtgcgtttgtctatatgttattttccaccatattgccgtcttttggcaatgtgagggcccggaaac
ctggccctgtcttcttgacgagcattcctaggggtctttcccctctcgccaaaggaatgcaaggtctgtt
gaatgtcgtgaaggaagcagttcctctggaagcttcttgaagacaaacaacgtctgtagcgaccctttgc
aggcagcggaaccccccacctggcgacaggtgcctctgcggccaaaagccacgtgtataagatacacctg
caaaggcggcacaaccccagtgccacgttgtgagttggatagttgtggaaagagtcaaatggctctcctc
aagcgtattcaacaaggggctgaaggatgcccagaaggtaccccattgtatgggatctgatctggggcct
cggtgcacatgctttacatgtgtttagtcgaggttaaaaaaacgtctaggccccccgaaccacggggacg
tggttttcctttgaaaaacacgatgataatatggccacaaccatgtcagtgagcaagggcgaggaggtca
tcaaagagttcatgcgcttcaaggtgcgcatggagggctccatgaacggccacgagttcgagatcgaggg
cgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggcggccccctg
cccttcgcctgggacatcctgtccccccagttcatgtacggctccaaggcgtacgtgaagcaccccgccg
acatccccgattacaagaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgagga
cggcggtctggtgaccgtgacccaggactcctccctgcaggacggcacgctgatctacaaggtgaagatg
cgcggcaccaacttcccccccgacggccccgtaatgcagaagaagaccatgggctgggaggcctccaccg
agcgcctgtacccccgcgacggcgtgctgaagggcgagatccaccaggccctgaagctgaaggacggcgg
ccactacctggtggagttcaagaccatctacatggccaagaagcccgtgcaactgcccggctactactac
gtggacaccaagctggacatcacctcccacaacgaggactacaccatcgtggaacagtacgagcgctccg
agggccgccaccacctgttcctgtacggcatggacgagctgtacaagtaaatcgataccgtcgacctcga
tcgagacctagaaaaacatggagcaatcacaagtagcaatacagcagctaccaatgctgattgtgcctgg
ctagaagcacaagaggaggaggaggtgggttttccagtcacacctcaggtacctttaagaccaatgactt
acaaggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactccca
acgaagacaagatatccttgatctgtggatctaccacacacaaggctacttccctgattggcagaactac
acaccagggccagggatcagatatccactgacctttggatggtgctacaagctagtaccagttgagcaag
agaaggtagaagaagccaatgaaggagagaacacccgcttgttacaccctgtgagcctgcatgggatgga
tgacccggagagagaagtattagagtggaggtttgacagccgcctagcatttcatcacatggcccgagag
ctgcatccggactgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactag
ggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtg
tgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagcatgtgagca
aaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgccccc
ctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagatacca
ggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtcc
gcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtagg
tcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaa
ctatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggatt
agcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaa
gaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatc
cggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaa
ggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaag
ggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaa
atcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatc
tcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacggg
agggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatc
agcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccag
tctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgcca
ttgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatc
aaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtc
agaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgc
catccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcg
accgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctc
atcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgt
aacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaac
aggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctt
tttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttaga
aaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgac
>21374 with mCherry-R 5′-TTGGTCACCTTCAGCTTGG
GCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCATGGAGCCCTCCATGCGCACCTTGAAGCGCATGAAC
TCTTTGATGACCTCCTCGCCCTTGCTCACTGACATGGTTGTGGCCATATTATCATCGTGTTTTTCAAAGG
AAAACCACGTCCCCGTGGTTCGGGGGGCCTAGACGTTTTTTTAACCTCGACTAAACACATGTAAAGCATG
TGTACCGAGGCCCCAGATCAGATCCCATACAATGGGGTACCTTCTGGGCATCCTTCAGCCCCTTGTTGAA
TACGCTTGAGGAGAGCCATTTGACTCTTTCCACAACTATCCAACTCACAACGTGGCACTGGGGTTGTGCC
GCCTTTGCAGGTGTATCTTATACACGTGGCTTTTGGCCGCAGAGGCACCTGTCGCCAGGTGGGGGGTTCC
GCTGCCTGCAAAGGGTCGCTACAGACGTTGTTTGTCTTCAAGAAGCTTCCAGAGGAACTGCTTCCTTCAC
GACATTCAACAGACCTTGCATTCCTTTGGCGAGAGGGGAAAGACCCCTAGGAATGCTCGTCAAGAAGACA
GGGCCAGGTTTCCGGGCCCTCACATTGCC
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文献和实验Production of Lentiviral Vector Supernatants and Transduction of Cellular Targets
particles. At top are the three plasmids pHIVPV, pHIV-TV, and the VSV G expression plasmid. A tri-transfection of 293T cells is performed by the calcium-phosphate technique. After 2–3 d, the supernatant is harvested (pseudotyped particles shown), and used
慢病毒转染肝细胞方法Lentivirus Transduction of Hematopoietic Cells
NaCl 2.7 mM KCl 8.1 mM Na2 HPO4 1.5 mM K2 H2 PO4 Plasmid DNA The plasmid DNAs in this protocol include pHIV7-GFP containing the desired transgenes
. no. 31985‐070) 0.5 to 1 µg/µl pHIV‐Luc‐ZsGreen (Addgene, cat. no. 39196) 0.5 to 1 µg/µl solution of pMDLg/pRRE 3rd‐generation
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