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- 详细信息
- 技术资料
- 保存条件：
负20度
- 英文名：
Recombinant Myosin Light Chain Kinase 3 (MYLK3)
- 库存：
大量
- 供应商：
柯雷生物
[ PROPERTIES ]
Residues: Gly488~Pro750 (Accession # Q3UIZ8),
with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Cytoplasm.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 6.0
Predicted Molecular Mass: 31.7kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-GYT VSQHEVLGGG RFGQVHRCTE RSTGLALAAK IIKVKNVKDR
EDVKNEVNIM NQLSHVNLIQ LYDAFESKSS FTLIMEYVDG GELFDRITDE KYHLTELDVV
LFTRQICEGV HYLHQHYILH LDLKPENILC VSQTGHQIKI IDFGLARRYK PREKLKVNFG
TPEFLAPEVV NYEFVSFPTD MWSVGVITYM LLSGLSPFLG ETDAETMNFI VNCSWDFDAD
TFKGLSEEAK DFVSRLLVKE KSCRMSATQC LKHEWLSHLP
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技术资料暂无技术资料 索取技术资料
肌球蛋白轻链激酶3(MYLK3)重组蛋白
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