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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant fusion protein containing a sequence corresponding to amino acids 35-276 of human KLK10 (NP_001070968.1).
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX33280
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
Kallikrein 10
- 抗体英文名:
Kallikrein 10 antibody
- 抗体名:
Kallikrein 10 抗体
- 规格:
100 μl
IHC-P analysis of human breast tissue using GTX33280 Kallikrein 10 antibody.
Dilution : 1:100
ICC/IF analysis of MCF7 cells using GTX33280 Kallikrein 10 antibody.
Blue : DAPI
WB analysis of various sample lysates using GTX33280 Kallikrein 10 antibody.
Dilution : 1:1000
Loading : 25μg per lane
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文献和实验RUVKUN Antibody Staining Protocol revised 10/29/90
RUVKUN Antibody Staining Protocol revised 10/29/90 Fixation: 1. C. elegans N2 grown in liquid or plates. Harvest worms. Wash for 2 hours at room temp. in 1X M9 or PBS. 2. Fix in 5 ml of FRESH THAT DAY 1% paraformaldehyde:
the antibody, load the affigel concentrate onto the column and hold at room temperature for 10 minutes.(15) Wash with 50 ml 10 mM Tris 7.5 and then wash with 50 ml 10 mM Tris 7.5/500 mM NaCl. Elute with 5 ml 100 mM Glycine pH 2.5 into 1 ml 1M Tris 8.0.(16
. The first is to Dialyze the coupled antibody against PBS for 24 hours with 3 changes of PBS. Doing this will not change concentration of original antibody (recommended for antibody with a concentration less than 1mg/mL). The second way is to use a PD-10 column. Using
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